Kim Soo-Jung, Kim Jin-Woo, Lee Ye-Gi, Park Yong-Cheol, Seo Jin-Ho
Center for Food and Bioconvergence, Seoul National University, Seoul, 08826, Republic of Korea.
Department of Bio and Fermentation Convergence Technology and BK21 Plus Program, Kookmin University, Seoul, 02707, Republic of Korea.
Appl Microbiol Biotechnol. 2017 Mar;101(6):2241-2250. doi: 10.1007/s00253-017-8172-1. Epub 2017 Feb 15.
Saccharomyces cerevisiae is a work horse for production of valuable biofuels and biochemicals including 2,3-butanediol (2,3-BDO), a platform chemical with wide industrial applications for synthetic rubber, biosolvents and food additives. Recently, a cutting-edge technology of metabolic engineering has enabled S. cerevisiae to produce 2,3-BDO with high yield and productivity. These include (i) amplification of the 2,3-BDO biosynthetic pathway, (ii) redirection of carbon flux from ethanol or glycerol toward 2,3-BDO, and (iii) 2,3-BDO production from sugars derived from renewable biomass. These breakthroughs enforced S. cerevisiae to become a promising microbial host for production of 2,3-BDO.
酿酒酵母是生产有价值生物燃料和生化制品的主力，这些制品包括2,3-丁二醇（2,3-BDO），这是一种在合成橡胶、生物溶剂和食品添加剂等领域有广泛工业应用的平台化学品。最近，代谢工程的一项前沿技术使酿酒酵母能够高产且高效地生产2,3-丁二醇。这些技术包括：（i）扩增2,3-丁二醇生物合成途径；（ii）将碳流从乙醇或甘油重定向至2,3-丁二醇；以及（iii）利用可再生生物质衍生的糖类生产2,3-丁二醇。这些突破促使酿酒酵母成为生产2,3-丁二醇的有前景的微生物宿主。
