Department of Endodontics, Dental School of Bauru, University of São Paulo, Bauru, Brazil.
Int Endod J. 2012 Feb;45(2):162-8. doi: 10.1111/j.1365-2591.2011.01959.x. Epub 2011 Oct 11.
To evaluate the residual biovolume of live bacterial cells, the mean biofilm thickness and the substratum coverage found in mixed biofilms treated with different endodontic irrigant solutions.
Twenty-five bovine dentine specimens were infected intraorally using a removable orthodontic device. Five samples were used for each irrigant solution: 2% chlorhexidine, 1% sodium hypochlorite (NaOCl), 10% citric acid, 17% EDTA and distilled water. The solutions were used for 5 min. The samples were stained using the Live/Dead technique and evaluated using a confocal microscope. Differences in the amount of total biovolume (μm(3)), number of surviving cells (μm(3)), mean biofilm thickness (μm) and substratum coverage (%) of the treated biofilms were determined using nonparametric statistical tests (P < 0.05).
Similar values of biovolume total, biovolume of live subpopulations and substratum coverage were found in 2% chlorhexidine, 10% citric acid, 17% EDTA and distilled water-treated biofilms (P > 0.05). The lower values of the studied parameters were found in 1% NaOCl-treated dentine (P < 0.05) with the exception of the mean biofilm height criteria that did not reveal significant differences amongst the irrigant solutions (P > 0.05).
One per cent sodium hypochlorite was the only irrigant that had a significant effect on biofilm viability and architecture.
评估不同根管冲洗液处理后的混合生物膜中活细菌细胞的剩余生物体积、平均生物膜厚度和基质覆盖率。
25 个牛牙本质标本通过可移动的正畸装置进行口腔内感染。每种冲洗液使用 5 个样本：2%洗必泰、1%次氯酸钠（NaOCl）、10%柠檬酸、17% EDTA 和蒸馏水。使用 5 分钟。使用 Live/Dead 技术对样本进行染色，并使用共聚焦显微镜进行评估。使用非参数统计检验（P < 0.05）确定处理生物膜的总生物体积（μm³）、存活细胞数量（μm³）、平均生物膜厚度（μm）和基质覆盖率（%）的差异。
2%洗必泰、10%柠檬酸、17% EDTA 和蒸馏水处理的生物膜中总生物体积、活细胞生物体积和基质覆盖率的数值相似（P > 0.05）。1% NaOCl 处理的牙本质中研究参数的值较低（P < 0.05），但冲洗液之间的平均生物膜高度标准没有显著差异（P > 0.05）。
1%次氯酸钠是唯一对生物膜活力和结构有显著影响的冲洗液。
