Department of Microbiology, School of Dentistry, University of Granada, Granada, Spain.
Department of Endodontics, Bauru Dental School, University of São Paulo, Bauru, Brazil.
Int Endod J. 2015 Dec;48(12):1188-93. doi: 10.1111/iej.12424. Epub 2015 Jan 8.
To evaluate the antimicrobial effect of 2.5% sodium hypochlorite alone (NaOCl) and associated with 9% HEBP (NaOCl/HEBP), 2% peracetic acid (PAA) and 2% chlorhexidine (CHX), on the viability of Enterococcus faecalis biofilms attached to dentine.
Biofilms of E. faecalis were grown on the surface of dentine blocks for 5 days and then exposed to the irrigating solutions for 3 min. Distilled water was used as the control. The total biovolume and the percentage of dead cells of the infected dentine were measured by means of confocal microscopy and the live/dead technique. Nonparametric tests were used to determine statistical differences (P < 0.05).
NaOCl and the NaOCl/HEBP mixture were associated with a significantly greater percentage of dead cells, followed by PAA (P < 0.05). No significant antimicrobial effect of CHX was observed in comparison with the control group. Total biovolume decreased significantly in NaOCl, NaOCl/HEBP and PAA solutions in comparison with the CHX and control groups.
NaOCl alone or associated with HEBP were the most effective irrigant solutions in dissolving and killing E. faecalis biofilms.
评估 2.5%次氯酸钠（NaOCl）单独使用和与 9%羟乙基纤维素（HEBP）（NaOCl/HEBP）、2%过氧乙酸（PAA）和 2%洗必泰（CHX）联合使用对黏附于牙本质的粪肠球菌生物膜的抗菌效果。
在牙本质块表面培养粪肠球菌生物膜 5 天，然后用冲洗液处理 3 分钟。蒸馏水作为对照。通过共聚焦显微镜和活/死技术测量感染牙本质的总生物量和死亡细胞的百分比。使用非参数检验确定统计学差异（P<0.05）。
NaOCl 和 NaOCl/HEBP 混合物与更高比例的死亡细胞相关，其次是 PAA（P<0.05）。与对照组相比，CHX 对粪肠球菌没有明显的抗菌作用。与 CHX 和对照组相比，NaOCl、NaOCl/HEBP 和 PAA 溶液中的总生物量显著减少。
单独使用 NaOCl 或与 HEBP 联合使用是溶解和杀死粪肠球菌生物膜最有效的冲洗液。
