Kruh G D, Perego R, Miki T, Aaronson S A
Laboratory of Cellular and Molecular Biology, National Cancer Institute, Bethesda, MD 20892.
Proc Natl Acad Sci U S A. 1990 Aug;87(15):5802-6. doi: 10.1073/pnas.87.15.5802.
We have previously described partial genomic sequences of arg, a human gene related to c-abl, and shown that it is expressed as a 12-kilobase transcript and is located at chromosome position 1q24-25. In this study we elucidate the complete coding sequence of arg by characterization of cDNA clones. Analysis of the predicted amino acid sequence of arg revealed that it is indeed closely related to that of c-abl. The two proteins are strikingly similar with regard to overall structural architecture as well as the amino acid sequences of their tyrosine kinase and src homologous 2 and 3 domains. In addition, arg, like c-abl, is expressed as two transcripts that result from a process of alternative splicing and encode alternative protein forms that differ only in their amino termini. The two genes define the Abelson subfamily of cytoplasmic tyrosine kinases and share a common homolog in Drosophila.
我们之前已经描述了arg的部分基因组序列，arg是一个与c-abl相关的人类基因，并表明它以12千碱基的转录本形式表达，位于染色体1q24-25位置。在本研究中，我们通过对cDNA克隆的表征阐明了arg的完整编码序列。对arg预测氨基酸序列的分析表明，它确实与c-abl的氨基酸序列密切相关。这两种蛋白质在整体结构架构以及酪氨酸激酶和src同源2和3结构域的氨基酸序列方面惊人地相似。此外，与c-abl一样，arg也以两种转录本形式表达，这两种转录本是由可变剪接过程产生的，编码的可变蛋白形式仅在其氨基末端有所不同。这两个基因定义了细胞质酪氨酸激酶的阿贝尔森亚家族，并且在果蝇中有一个共同的同源物。
