Henkemeyer M J, Bennett R L, Gertler F B, Hoffmann F M
McArdle Laboratory for Cancer Research, University of Wisconsin, Madison 53706.
Mol Cell Biol. 1988 Feb;8(2):843-53. doi: 10.1128/mcb.8.2.843-853.1988.
We report our molecular characterization of the Drosophila melanogaster Abelson gene (abl), a gene in which recessive loss-of-function mutations result in lethality at the pupal stage of development. This essential gene consists of 10 exons extending over 26 kilobase pairs of genomic DNA. The DNA sequence encodes a protein of 1,520 amino acids with strong sequence similarity to the human c-abl proto-oncogene beginning in the type lb 5' exon and extending through the region essential for tyrosine kinase activity. When the tyrosine kinase homologous region was expressed in Escherichia coli, phosphorylation of proteins on tyrosine residues was observed with an antiphosphotyrosine antibody. These results show that the abl gene is highly conserved through evolution and encodes a functional tyrosine protein kinase required for Drosophila development.
我们报告了对果蝇黑腹Abelson基因（abl）的分子特征分析，该基因的隐性功能丧失突变会导致发育蛹期致死。这个必需基因由10个外显子组成，跨越26千碱基对的基因组DNA。该DNA序列编码一个由1520个氨基酸组成的蛋白质，从1b型5'外显子开始，与人类c-abl原癌基因具有很强的序列相似性，并延伸至酪氨酸激酶活性所必需的区域。当酪氨酸激酶同源区域在大肠杆菌中表达时，用抗磷酸酪氨酸抗体观察到蛋白质酪氨酸残基的磷酸化。这些结果表明，abl基因在进化过程中高度保守，并且编码果蝇发育所需的功能性酪氨酸蛋白激酶。
