University College Cork, Alimentary Pharmabiotic Centre, College Road, Cork, Ireland.
Benef Microbes. 2011 Sep;2(3):199-207. doi: 10.3920/BM2011.0015.
Brain-derived neurotrophic factor (BDNF) is of interest because of its putative role in stress and psychiatric disorders. Maternal separation is used as an animal model of early-life stress and of irritable bowel syndrome (IBS). Animals exposed to the paradigm show altered gut function together with heightened levels of arousal and corticosterone. Some probiotic organisms have been shown to be of benefit in IBS and influence the brain-gut axis. Our objective was to investigate the effects of maternal separation on BDNF under basal conditions and in response to the probiotic Bifidobacterium breve 6330. The study implemented the maternal separation model which we have previously described. Polymerase chain reaction and in situ hybridisation were performed to measure the effect of maternal separation on both BDNF total variants and BDNF splice variant (exon) IV in the hippocampus. Maternally separated and non-separated rats were treated with B. breve 6330, to investigate the effect of this probiotic on BDNF total variant and BDNF exon IV expression. Maternal separation increased BDNF total variants (P<0.01), whilst having no effect on BDNF exon IV. B. breve 6330 increased BDNF total variants (P<0.01), and decreased BDNF splice variant IV, in non-separated rats (P<0.01). B. breve 6330 did not alter BDNF levels in the maternally separated rats. Maternal separation caused a marked increase in BDNF in the hippocampus. While B. breve 6330 influenced BDNF in normal animals, it had no significant effect on BDNF in those which were maternally separated. We have demonstrated that an orally administered probiotic can influence hippocampal BDNF.
脑源性神经营养因子（BDNF）因其在应激和精神疾病中的作用而备受关注。母体分离被用作动物模型来模拟早期生活压力和肠易激综合征（IBS）。暴露于该模型的动物表现出肠道功能改变，同时觉醒水平和皮质酮水平升高。一些益生菌已被证明对 IBS 有益，并影响脑-肠轴。我们的目的是研究在基础条件下和在益生菌短双歧杆菌 6330 的作用下，母体分离对 BDNF 的影响。该研究实施了我们之前描述的母体分离模型。聚合酶链反应和原位杂交用于测量母体分离对海马体中 BDNF 总变体和 BDNF 剪接变体（外显子）IV 的影响。对分离和未分离的大鼠进行 B. breve 6330 治疗，以研究这种益生菌对 BDNF 总变体和 BDNF 外显子 IV 表达的影响。母体分离增加了 BDNF 总变体（P<0.01），而对 BDNF 外显子 IV 没有影响。B. breve 6330 增加了未分离大鼠的 BDNF 总变体（P<0.01），并减少了 BDNF 剪接变体 IV（P<0.01）。B. breve 6330 未改变母体分离大鼠的 BDNF 水平。母体分离导致海马体中 BDNF 明显增加。虽然 B. breve 6330 影响正常动物的 BDNF，但对母体分离的动物没有显著影响。我们已经证明，口服益生菌可以影响海马体中的 BDNF。
