Bacterial Discovery Laboratory, Centre for Environment, IST, JNT University Hyderabad, Kukatpally, Hyderabad 500 085, India.
Department of Plant Sciences, School of Life Sciences, University of Hyderabad, PO Central University, Hyderabad 500 046, India.
Int J Syst Evol Microbiol. 2013 Jan;63(Pt 1):200-207. doi: 10.1099/ijs.0.038620-0. Epub 2012 Mar 2.
Two strains (JA575(T) and JA576(T)) of orange- to pink-pigmented, rod-shaped, motile and budding phototrophic bacteria were isolated from paddy soils. Both strains contained bacteriochlorophyll a and carotenoids of spirilloxanthin series. Both strains had C(18 : 1)ω7c as the major cellular fatty acid, ubiquinone-10 (Q(10)) as the main quinone, and diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and phosphatidylcholine as polar lipids. Phylogenetic analysis on the basis of 16S rRNA gene sequences showed that both strains clustered with species of the genus Rhodopseudomonas in the class Alphaproteobacteria. Strains JA575(T) and JA576(T) were genotypically (<35 % DNA-DNA relatedness) and phenotypically distinct from each other. Further, both strains showed less than 48 % DNA-DNA relatedness with the type strains of all recognized species of the genus Rhodopseudomonas. The molecular evidence is supported by phenotypic evidence. It is proposed that strains JA575(T) and JA576(T) be classified as representing two novel species of the genus Rhodopseudomonas with the species names Rhodopseudomonas pentothenatexigens sp. nov. and Rhodopseudomonas thermotolerans sp. nov., respectively. The type strains of the proposed novel species are JA575(T) (= NBRC 108862(T) = KCTC15143(T)) and JA576(T) (= NBRC 108863(T) = KCTC 15144(T)), respectively.
两株（JA575(T) 和 JA576(T)）橙色至粉红色着色、杆状、运动和芽殖光养细菌从稻田土壤中分离得到。两株菌均含有细菌叶绿素 a 和螺旋藻叶黄素系列的类胡萝卜素。两株菌的主要细胞脂肪酸均为 C(18 : 1)ω7c，主要醌为泛醌-10 (Q(10))，极性脂类为二磷脂酰甘油、磷脂酰甘油、磷脂酰乙醇胺和磷脂酰胆碱。基于 16S rRNA 基因序列的系统发育分析表明，两株菌均与α变形菌纲红假单胞菌属的种聚在一起。菌株 JA575(T) 和 JA576(T) 在基因型（<35 % DNA-DNA 同源性）和表型上彼此不同。此外，两株菌与所有公认的红假单胞菌属种的模式株之间的 DNA-DNA 同源性均小于 48 %。分子证据得到表型证据的支持。建议将菌株 JA575(T) 和 JA576(T) 分别归类为代表红假单胞菌属的两个新种，其种名分别为戊糖营养红假单胞菌（Rhodopseudomonas pentothenatexigens）sp. nov. 和耐热红假单胞菌（Rhodopseudomonas thermotolerans）sp. nov.。提议的新种的模式株分别为 JA575(T)（= NBRC 108862(T) = KCTC15143(T)）和 JA576(T)（= NBRC 108863(T) = KCTC 15144(T)）。
