Department of Dermatology, University of Regensburg, 93042, Regensburg, Germany.
Photochem Photobiol Sci. 2012 Jan;11(1):107-17. doi: 10.1039/c1pp05142c. Epub 2011 Oct 11.
UVA irradiation (320-400 nm) comprises about 95 percent of incident midday solar ultraviolet irradiation. It penetrates skin much deeper than UVB irradiation. The absorption of UVA irradiation in endogenous chromophores frequently leads to the generation of reactive oxygen species such as singlet oxygen ((1)O(2)). (1)O(2) is an important biochemical intermediate in multiple biological processes. Beside other procedures, the direct detection of (1)O(2) by its luminescence is a powerful tool that helps to understand the generation of (1)O(2) during UVA exposure in solution, in vitro and in vivo. This article describes the endogenous photosensitizers, their ability to generate (1)O(2) under UVA irradiation, and the detection technology to visualize the action of (1)O(2).
UVA 辐射（320-400nm）约占正午太阳紫外线照射的 95%。它比 UVB 辐射穿透皮肤更深。内源性生色团对 UVA 辐射的吸收经常导致活性氧物质的产生，如单线态氧（(1)O(2)）。(1)O(2)是多种生物过程中的重要生化中间体。除其他程序外，通过其发光直接检测(1)O(2)是一种强大的工具，有助于理解在溶液、体外和体内 UVA 暴露期间(1)O(2)的产生。本文描述了内源性光敏剂，它们在 UVA 辐射下产生(1)O(2)的能力，以及可视化(1)O(2)作用的检测技术。
