Arakane K, Ryu A, Hayashi C, Masunaga T, Shinmoto K, Mashiko S, Nagano T, Hirobe M
Research Laboratory, Kosé Corporation, Tokyo, Japan.
Biochem Biophys Res Commun. 1996 Jun 25;223(3):578-82. doi: 10.1006/bbrc.1996.0937.
Although singlet oxygen has been postulated to be a highly reactive and toxic intermediate, there has been no evidence of considerable generation of singlet oxygen in vivo level except for special cases. In this work, we firstly measured the near-infrared emission spectra corresponding to the O2(1 delta g) --> O2(3 epsilon g-) transition of singlet oxygen of cutaneous Propionibacterium acnes (P. acnes) porphyrin under laser excitation. A comparison of the singlet oxygen production of coproporphyrin, which is produced predominantly from P. acnes, with that of other photosensitizers revealed coproporphyrin to be a highly efficient singlet oxygen generator under ultraviolet light A irradiation on the skin. These results suggest that singlet oxygen can be generated on the skin surface from P. acnes porphyrin under ultraviolet irradiation and induce serious damage to the skin.
尽管单线态氧被假定为一种高活性且有毒的中间体，但除特殊情况外，尚无证据表明在体内水平会大量生成单线态氧。在本研究中，我们首先测量了在激光激发下，皮肤痤疮丙酸杆菌（P. acnes）卟啉的单线态氧从O2(1Δg)跃迁到O2(3Σg-)时对应的近红外发射光谱。将主要由痤疮丙酸杆菌产生的粪卟啉与其他光敏剂的单线态氧生成情况进行比较，结果显示在皮肤接受紫外线A照射时，粪卟啉是一种高效的单线态氧生成剂。这些结果表明，在紫外线照射下，痤疮丙酸杆菌卟啉可在皮肤表面生成单线态氧，并对皮肤造成严重损伤。
