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南极地衣甲醇提取物对脂多糖刺激的 RAW264.7 巨噬细胞和斑马鱼模型的抗炎作用。

Anti-Inflammatory Effects of Antarctic Lichen Methanol Extract in Lipopolysaccharide-Stimulated RAW 264.7 Macrophage Cells and Zebrafish Model.

机构信息

Division of Life Sciences, Korea Polar Research Institute, Incheon 21990, Republic of Korea.

Department of Pharmacy, Sungkyunkwan University, Suwon 16419, Republic of Korea.

出版信息

Biomed Res Int. 2021 Feb 16;2021:8812090. doi: 10.1155/2021/8812090. eCollection 2021.

DOI:10.1155/2021/8812090
PMID:33644231
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7902135/
Abstract

(UA) is a member of the family Umbilicariaceae. To the best of our knowledge, no studies on its anti-inflammatory effects have been reported yet. In the present study, we examined its ability to suppress inflammatory responses and the molecular mechanisms underlying these abilities using lipopolysaccharide- (LPS-) stimulated RAW 264.7 cells and a zebrafish model of inflammation. We investigated the effects of UA on the production of nitric oxide (NO) and prostaglandin E (PGE) in LPS-stimulated RAW 264.7 cells. To explore the anti-inflammatory mechanisms of UA, we measured the mRNA and protein expression of proinflammatory mediators in LPS-stimulated RAW 264.7 cells using quantitative RT-PCR and western blot analyses, respectively. UA significantly inhibited the production of NO, PGE, interleukin- (IL-) 6, and tumor necrosis factor- (TNF-) in the LPS-stimulated RAW 264.7 cells. It also suppressed the mRNA and protein expression of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), and nuclear factor- (NF-) B activation in LPS-stimulated RAW 264.7 cells and tail pin-cutting-induced zebrafish model. Collectively, these findings indicate that UA significantly inhibits LPS-stimulated inflammatory responses. These effects were considered to be strongly associated with the suppression of NF-B activation. Overall, our results demonstrate that UA extract exerts strong anti-inflammatory activities in and models and suggest that UA may be an effective novel therapeutic agent for the treatment of inflammatory diseases.

摘要

(UA) 属于 Umbilicariaceae 科。据我们所知，目前尚未有关于其抗炎作用的研究报道。在本研究中，我们使用脂多糖 (LPS) 刺激的 RAW 264.7 细胞和炎症斑马鱼模型，研究了其抑制炎症反应的能力及其潜在的分子机制。我们研究了 UA 对 LPS 刺激的 RAW 264.7 细胞中一氧化氮 (NO) 和前列腺素 E (PGE) 产生的影响。为了探讨 UA 的抗炎机制，我们使用定量 RT-PCR 和 Western blot 分析分别测量了 LPS 刺激的 RAW 264.7 细胞中促炎介质的 mRNA 和蛋白表达。UA 显著抑制了 LPS 刺激的 RAW 264.7 细胞中 NO、PGE、白细胞介素- (IL-) 6 和肿瘤坏死因子- (TNF-) 的产生。它还抑制了 LPS 刺激的 RAW 264.7 细胞中诱导型一氧化氮合酶 (iNOS)、环氧化酶-2 (COX-2) 和核因子- (NF-) B 激活的 mRNA 和蛋白表达，以及尾部夹伤诱导的斑马鱼模型。综上所述，这些发现表明 UA 可显著抑制 LPS 刺激的炎症反应。这些作用被认为与 NF-B 激活的抑制密切相关。总的来说，我们的研究结果表明 UA 提取物在 和 模型中具有强大的抗炎活性，并提示 UA 可能是治疗炎症性疾病的有效新型治疗剂。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/69f6/7902135/553a3d1da979/BMRI2021-8812090.009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/69f6/7902135/8a6f7b2a7ae4/BMRI2021-8812090.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/69f6/7902135/8135fa3c8669/BMRI2021-8812090.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/69f6/7902135/830997e7ea9d/BMRI2021-8812090.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/69f6/7902135/0571b89a7150/BMRI2021-8812090.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/69f6/7902135/ffc146df10fb/BMRI2021-8812090.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/69f6/7902135/0571499c33e4/BMRI2021-8812090.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/69f6/7902135/69153b959d0c/BMRI2021-8812090.007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/69f6/7902135/68489ea67833/BMRI2021-8812090.008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/69f6/7902135/553a3d1da979/BMRI2021-8812090.009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/69f6/7902135/8a6f7b2a7ae4/BMRI2021-8812090.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/69f6/7902135/8135fa3c8669/BMRI2021-8812090.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/69f6/7902135/830997e7ea9d/BMRI2021-8812090.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/69f6/7902135/0571b89a7150/BMRI2021-8812090.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/69f6/7902135/ffc146df10fb/BMRI2021-8812090.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/69f6/7902135/0571499c33e4/BMRI2021-8812090.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/69f6/7902135/69153b959d0c/BMRI2021-8812090.007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/69f6/7902135/68489ea67833/BMRI2021-8812090.008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/69f6/7902135/553a3d1da979/BMRI2021-8812090.009.jpg

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