Alpsoy L, Yildirim A, Agar G
Fatih University, Faculty of Art and Science, Department of Biology, Istanbul, Turkey.
Toxicol Ind Health. 2009 Mar;25(2):121-7. doi: 10.1177/0748233709103413.
The aim of this study was to investigate the possible protective role of vitamin A, C, and E on aflatoxin B(1)-induced in human lymphocytes using biochemical approaches. The control group received dimethyl sulfoxide, the second group of cultures were administered aflatoxin B(1) (AFB(1)) at a dose of 5 muM. The other group of cultures were treated with AFB(1)+vitamin A (0.5 and 1.0 and 1.5 microM) and AFB(1)+vitamin C (25, 50, and 100 microM) and AFB(1)+vitamin E (40, 100, and 200 microM). The results of this experiment show that AFB(1) significantly decreased the level of GSH and the activities of superoxide dismutase and GPx and increased level of malondialdehyde. Simultaneous supplementation with vitamin A, C, and E restored these parameters to that of normal range. In conclusion, vitamin A, C, and E exhibited protective effects in human lymphocytes by inhibiting AFB(1)-induced ROS generation.
本研究的目的是采用生化方法探讨维生素A、C和E对黄曲霉毒素B(1)诱导人淋巴细胞的可能保护作用。对照组接受二甲基亚砜，第二组培养物给予剂量为5 μM的黄曲霉毒素B(1)（AFB(1)）。另一组培养物分别用AFB(1)+维生素A（0.5、1.0和1.5 μM）、AFB(1)+维生素C（25、50和100 μM）以及AFB(1)+维生素E（40、100和200 μM）处理。该实验结果表明，AFB(1)显著降低了谷胱甘肽水平、超氧化物歧化酶和谷胱甘肽过氧化物酶的活性，并提高了丙二醛水平。同时补充维生素A、C和E可将这些参数恢复至正常范围。总之，维生素A、C和E通过抑制AFB(1)诱导的活性氧生成，对人淋巴细胞发挥了保护作用。
