智利丙型肝炎慢性患者的治疗反应与 IL28B 多态性相关。
IL28B polymorphisms associated with therapy response in Chilean chronic hepatitis C patients.
机构信息
Centro de Estudios Avanzados de las Hepatitis Virales, Sección de Gastroenterología, Departamento de Medicina, Hospital Clínico Universidad de Chile, 8340457 Santiago, Chile.
出版信息
World J Gastroenterol. 2011 Aug 21;17(31):3636-9. doi: 10.3748/wjg.v17.i31.3636.
AIM
To analyze the association of three IL28B single nucleotide polymorphisms with response to therapy in Chilean patients infected with hepatitis C virus (HCV).
METHODS
We studied two groups of patients with chronic HCV infection (genotype 1), under standard combined treatment with pegylated interferon plus ribavirin. One group consisted of 50 patients with sustained virological response, whereas the second group consisted of 49 null responders. In order to analyze the IL28B single nucleotide polymorphisms rs12979860, rs12980275 and rs8099917, samples were used for polymerase chain reaction amplification, and the genotyping was performed by restriction fragment length polymorphism.
RESULTS
The IL28B rs12979860 CC, rs12980275 AA and rs8099917 TT genotypes were much more frequently found in patients with sustained virological response compared to null responders (38%, 44% and 50% vs 2%, 8.2% and 8.2%, respectively). These differences were highly significant in all three cases (P < 0.0001).
CONCLUSION
The three IL28B polymorphisms studied are strongly associated with sustained virological response to therapy in Chilean patients with chronic HCV (genotype 1).
目的
分析智利丙型肝炎病毒（HCV）感染患者中三种 IL28B 单核苷酸多态性与治疗反应的相关性。
方法
我们研究了两组慢性 HCV 感染（基因型 1）的患者，他们接受聚乙二醇干扰素联合利巴韦林的标准联合治疗。一组包括 50 例持续病毒学应答患者，另一组包括 49 例无应答者。为了分析 IL28B 单核苷酸多态性 rs12979860、rs12980275 和 rs8099917，使用聚合酶链反应扩增样本，并通过限制性片段长度多态性进行基因分型。
结果
与无应答者相比，持续病毒学应答患者中 IL28B rs12979860 CC、rs12980275 AA 和 rs8099917 TT 基因型更为常见（分别为 38%、44%和 50% vs 2%、8.2%和 8.2%）。这三种情况的差异均具有统计学显著性（均 P < 0.0001）。
结论
在所研究的三种 IL28B 多态性中，智利慢性 HCV（基因型 1）患者对治疗的持续病毒学应答与它们密切相关。
Zotero 插件