用烷化剂消融小鼠空肠隐窝
Ablation of murine jejunal crypts by alkylating agents.
作者信息
Moore J V
出版信息
Br J Cancer. 1979 Feb;39(2):175-81. doi: 10.1038/bjc.1979.28.
The gut microcolony assay has been used to measure damage to intestinal crypts by single and split doses of 3 alkylating agents: mechlorethamine hydrochloride (HN2), bis-chloroethyl-nitrosourea (BCNU) and isopropyl methane sulphonate (IMS). The single-dose survival curves for whole crypts were distinguished by extrapolation numbers (3.0, 176 and 1.5 respectively) that were lower than most previously published values for assay by irradiation. Significant sparing of crypts occurred when doses of HN2 or BCNU, but not IMS, were given in 2 equal fractions separated by more than 2 h. Deduced D0 values for those cells from which crypts regenerate were 1.9 mg/kg HN2, 19 mg/kg BCNU and 487 mg/kg IMS.
肠道微克隆试验已被用于测定3种烷化剂单剂量和分剂量对肠隐窝的损伤，这3种烷化剂分别是盐酸氮芥（HN2）、双氯乙基亚硝脲（BCNU）和甲磺酸异丙酯（IMS）。全隐窝的单剂量存活曲线通过外推数（分别为3.0、176和1.5）来区分，这些外推数低于此前大多数已发表的辐射试验值。当HN2或BCNU（而非IMS）的剂量以2个等份给予，且间隔超过2小时时，隐窝出现显著的存活。隐窝再生所源自的那些细胞的推导D0值分别为：HN2 1.9 mg/kg、BCNU为19 mg/kg、IMS为487 mg/kg。
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