CAS Key Lab of Health Informatics, Institute of Biomedical and Health Engineering, Chinese Academy of Sciences, Shenzhen, 518055, Guangdong, PR China.
Nanoscale. 2011 Nov;3(11):4724-32. doi: 10.1039/c1nr10933b. Epub 2011 Oct 11.
Highly luminescent near-infrared (NIR) emitting CdTe/CdSe quantum dots (QDs) were prepared through a fast and convenient method, and a new type of multivalent polymer ligands was used as the surface substituents to prepare highly stable hydrophilic QDs with small sizes. The well-defined CdTe/CdSe QDs were characterized by transmission electron microscopy (TEM), X-ray powder diffraction (XRD), energy dispersive X-ray (EDX) spectroscopy and photoluminescence (PL) spectroscopy, respectively. The as-prepared CdTe/CdSe QDs were photostable with high PL quantum yields (QYs) (up to 66% at room temperature), low toxicity to cells at experimental dosages, and the QDs' fluorescence emissions were tunable between 700 and 820 nm. Furthermore, fluorescence imaging using CdTe/CdSe QDs conjugated with the AS1411 aptamer (targeting nucleolin) probe in cancer cells was reported, and the CdTe/CdSe QDs were also successfully applied for the fluorescence imaging of living animals. Our preliminary results illustrated that the CdTe/CdSe NIR-QDs with small sizes would be an alternative probe for ultrasensitive, multicolor, and multiplex applications, especially for in vivo imaging applications.
通过一种快速简便的方法制备了具有高光致发光性能的近红外（NIR）发射 CdTe/CdSe 量子点（QDs），并使用新型多价聚合物配体作为表面取代基，制备了具有小尺寸和高稳定性的亲水性 QDs。通过透射电子显微镜（TEM）、X 射线粉末衍射（XRD）、能谱（EDX）和光致发光（PL）光谱分别对合成的 CdTe/CdSe QDs 进行了表征。所制备的 CdTe/CdSe QDs 具有光稳定性，PL 量子产率高（室温下高达 66%），在实验剂量下对细胞的毒性低，并且 QDs 的荧光发射可在 700nm 至 820nm 之间调谐。此外，还报道了用与核仁素（nucleolin）靶标 AS1411 适配体（aptamer）探针偶联的 CdTe/CdSe QDs 在癌细胞中进行荧光成像，并且成功地将 CdTe/CdSe QDs 应用于活体动物的荧光成像。我们的初步结果表明，具有小尺寸的 CdTe/CdSe NIR-QDs 将成为超灵敏、多色和多重应用的替代探针，特别是在体内成像应用中。
