Chen Zhi-Liang, Yu Zi-Li, Zhou Miao-Miao, Zhang Song, Zhang Bing, Liu Yi, Zhao Yi-Fang, Cao Hui-Min, Lin Yi, Zhang Zhi-Ling, Pang Dai-Wen
College of Chemistry and Molecular Sciences, State Key Laboratory of Virology, The Institute for Advanced Studies, and Wuhan Institute of Biotechnology, Wuhan University, Wuhan 430072, P. R. China.
School of Pharmacy, Shaoyang University, Shaoyang 422000, P. R. China.
ACS Omega. 2020 Jun 9;5(24):14261-14266. doi: 10.1021/acsomega.9b04081. eCollection 2020 Jun 23.
Near-infrared (NIR) fluorescence has attracted much attention in biomedical fields because it offers deep tissue penetration and high spatial resolution. Herein, a method is developed for the preparation of NIR fluorescent nanocomposites (NCs) by encapsulating natural chlorophyll (Chl) into the micelles of octylamine-modified poly(acrylic acid) (OPA). Both femtosecond transient absorption spectra and isothermal titration calorimetry thermogram reveal that the micelles of OPA provide a hydrophobic environment for the improved fluorescence efficiency. Hence the resulted Chl NCs possess unique properties such as ultrasmall size, outstanding photostability, good biocompatibility, and superbright NIR fluorescence emission. In vivo imaging of sentinel lymph node is achieved in nude mice, demonstrating the potential of Chl NCs in biomedical applications. This work provides a new strategy for the preparation of highly biocompatible NIR fluorescence labeling nanocomposites.
近红外（NIR）荧光因其具有深层组织穿透性和高空间分辨率而在生物医学领域备受关注。在此，通过将天然叶绿素（Chl）封装到辛胺改性聚丙烯酸（OPA）的胶束中，开发了一种制备近红外荧光纳米复合材料（NCs）的方法。飞秒瞬态吸收光谱和等温滴定量热法热谱图均表明，OPA胶束为提高荧光效率提供了疏水环境。因此，所得的Chl NCs具有诸如超小尺寸、出色的光稳定性、良好的生物相容性和超亮近红外荧光发射等独特性能。在裸鼠体内实现了前哨淋巴结的成像，证明了Chl NCs在生物医学应用中的潜力。这项工作为制备具有高度生物相容性的近红外荧光标记纳米复合材料提供了一种新策略。
