"Aurel Vlaicu" University, 2-4 Elena Dragoi str., 310330 Arad, Romania.
Appl Biochem Biotechnol. 2011 Dec;165(7-8):1789-98. doi: 10.1007/s12010-011-9395-8. Epub 2011 Oct 12.
A coordinatively immobilized laccase was prepared using a new cryogel type carrier. The support has a wide-pore texture facilitating diffusion of different substrates to the enzyme reaction center. The biocatalyst proved to be efficient in decolorization of two anthraquinone derivatives, namely Acid Blue 62 and bromaminic acid. After 24 h over 80% of the two substrates have been oxidated. The kinetic data (K (m) and V (max)) for the oxidation of the two anthraquinone derivatives, with the free and immobilized enzyme, have been determined and compared. Other parameters, like k (cat) and the specificity constant have been calculated and analyzed. The influence of substrate properties (hydrophobicity, polarity, etc.) has been discussed.
一种新的凝胶载体被用于制备配位固定化漆酶。这种载体具有大孔结构，有利于不同底物扩散至酶反应中心。该生物催化剂在两种蒽醌衍生物（酸性蓝 62 和溴氨酸）的脱色中表现出高效性。24 小时后，两种底物中有超过 80%被氧化。测定并比较了游离酶和固定化酶对两种蒽醌衍生物氧化的动力学参数（K (m) 和 V (max)）。还计算和分析了 k (cat)和比活性常数等其他参数。讨论了底物性质（疏水性、极性等）的影响。
