D'Arpa A, Accardo-Palumbo A, Amato G, D'Amelio L, Napoli B, Pileri D, Cataldo V, Mogavero R, Lombardo C, Conte F
Plastic Surgery and Burns Therapy Operating Unit, ARNAS, Civic Hospital, Palermo, Italy.
Ann Burns Fire Disasters. 2007 Dec 31;20(4):199-202.
Burn injury is associated with immune suppression and the subsequent development of sepsis. Severe burn injury is associated with depressed immune response, including a functional impairment of Th1 lymphocytes and natural killer cells and a decrease in interferon-a production. Dendritic cells (DCs) are potent antigen-presenting cells and play a key role in T cell activation; they are essential in coordinating the host response to pathogens. Using three-colour flow cytometry, we determined the percentage of lineage-negative LIN-DR+ DCs in burn patients and healthy subjects. The percentages of DCs were lower in the circulation of septic than in nonseptic patients and healthy subjects at all times examined (14 days) after burn injury. In contrast, the DC percentage in nonseptic patients was low at day 1, increased from day 3 to day 10, and reverted to normal levels at day 14. The data from the present study suggest that the DC percentage decreased early after burn injury. In addition, in the presence of severe sepsis, the DC percentage remained lower until day 14. This DC reduction may contribute to the immunosuppression observed after burn injury.
烧伤与免疫抑制及随后发生的脓毒症相关。严重烧伤与免疫反应抑制有关，包括Th1淋巴细胞和自然杀伤细胞功能受损以及干扰素-α产生减少。树突状细胞（DCs）是强大的抗原呈递细胞，在T细胞活化中起关键作用；它们对于协调宿主对病原体的反应至关重要。我们使用三色流式细胞术测定了烧伤患者和健康受试者中谱系阴性LIN-DR+ DCs的百分比。在烧伤后所有检查时间点（14天），脓毒症患者循环中的DC百分比均低于非脓毒症患者和健康受试者。相比之下，非脓毒症患者的DC百分比在第1天较低，从第3天到第10天增加，并在第14天恢复到正常水平。本研究数据表明，烧伤后早期DC百分比降低。此外，在严重脓毒症存在的情况下，DC百分比在第14天之前一直较低。这种DC减少可能导致烧伤后观察到的免疫抑制。
