British Columbia Cancer Agency Research Centre, Vancouver, British Columbia, Canada.
PLoS One. 2011;6(10):e25915. doi: 10.1371/journal.pone.0025915. Epub 2011 Oct 3.
Once thought to be a part of the 'dark matter' of the genome, long non-coding RNAs (lncRNAs) are emerging as an integral functional component of the mammalian transcriptome. LncRNAs are a novel class of mRNA-like transcripts which, despite no known protein-coding potential, demonstrate a wide range of structural and functional roles in cellular biology. However, the magnitude of the contribution of lncRNA expression to normal human tissues and cancers has not been investigated in a comprehensive manner. In this study, we compiled 272 human serial analysis of gene expression (SAGE) libraries to delineate lncRNA transcription patterns across a broad spectrum of normal human tissues and cancers. Using a novel lncRNA discovery pipeline we parsed over 24 million SAGE tags and report lncRNA expression profiles across a panel of 26 different normal human tissues and 19 human cancers. Our findings show extensive, tissue-specific lncRNA expression in normal tissues and highly aberrant lncRNA expression in human cancers. Here, we present a first generation atlas for lncRNA profiling in cancer.
曾经被认为是基因组“暗物质”的一部分，长链非编码 RNA（lncRNA）正在成为哺乳动物转录组的一个不可或缺的功能组成部分。lncRNA 是一类新型的 mRNA 样转录本，尽管没有已知的蛋白质编码潜力，但在细胞生物学中表现出广泛的结构和功能作用。然而，lncRNA 表达对正常人体组织和癌症的贡献程度尚未以全面的方式进行研究。在这项研究中，我们汇编了 272 个人类序列分析基因表达（SAGE）文库，以描绘广泛的正常人体组织和癌症中的 lncRNA 转录模式。我们使用一种新的 lncRNA 发现管道，解析了超过 2400 万个 SAGE 标签，并报告了 26 种不同正常人体组织和 19 种人类癌症中的 lncRNA 表达谱。我们的研究结果表明，在正常组织中存在广泛的组织特异性 lncRNA 表达，而在人类癌症中则存在高度异常的 lncRNA 表达。在这里，我们提出了癌症中 lncRNA 分析的第一代图谱。
