Leung Regina Won Kay, Yeh Shu-Chi Allison, Fang Qiyin
Biomed Opt Express. 2011 Sep 1;2(9):2517-31. doi: 10.1364/BOE.2.002517. Epub 2011 Aug 2.
Fluorescence lifetime imaging has emerged as an important microscopy technique, where high repetition rate lasers are the primary light sources. As fluorescence lifetime becomes comparable to intervals between consecutive excitation pulses, incomplete fluorescence decay from previous pulses can superimpose onto the subsequent decay measurements. Using a mathematical model, the incomplete decay effect has been shown to lead to overestimation of the amplitude average lifetime except in mono-exponential decays. An inverse model is then developed to correct the error from this effect and the theoretical simulations are tested by experimental results.
荧光寿命成像已成为一种重要的显微镜技术，其中高重复率激光器是主要光源。当荧光寿命与连续激发脉冲之间的间隔相当时，前一个脉冲的不完全荧光衰减会叠加到后续的衰减测量中。使用数学模型表明，除了单指数衰减外，不完全衰减效应会导致幅度平均寿命的高估。然后开发了一个逆模型来校正这种效应产生的误差，并通过实验结果对理论模拟进行了测试。
