荧光寿命成像显微镜在脑肿瘤图像引导手术中的应用。
Fluorescence lifetime imaging microscopy for brain tumor image-guided surgery.
机构信息
University of California, Davis, Department of Biomedical Engineering, Davis, California 95616, USA.
出版信息
J Biomed Opt. 2010 Sep-Oct;15(5):056022. doi: 10.1117/1.3486612.
We demonstrate for the first time the application of an endoscopic fluorescence lifetime imaging microscopy (FLIM) system to the intraoperative diagnosis of glioblastoma multiforme (GBM). The clinically compatible FLIM prototype integrates a gated (down to 0.2 ns) intensifier imaging system with a fiber-bundle (fiber image guide of 0.5 mm diameter, 10,000 fibers with a gradient index lens objective 0.5 NA, and 4 mm field of view) to provide intraoperative access to the surgical field. Experiments conducted in three patients undergoing craniotomy for tumor resection demonstrate that FLIM-derived parameters allow for delineation of tumor from normal cortex. For example, at 460±25-nm wavelength band emission corresponding to NADH/NADPH fluorescence, GBM exhibited a weaker fluorescence intensity (35% less, p-value<0.05) and a longer lifetime τGBM-Amean=1.59±0.24 ns than normal cortex τNC-Amean=1.28±0.04 ns (p-value<0.005). Current results demonstrate the potential use of FLIM as a tool for image-guided surgery of brain tumors.
我们首次展示了内窥镜荧光寿命成像显微镜（FLIM）系统在多形性胶质母细胞瘤（GBM）术中诊断中的应用。该临床兼容的 FLIM 原型集成了门控（低至 0.2 ns）增强成像系统和光纤束（光纤图像引导器直径为 0.5 毫米，10000 根光纤，梯度指数透镜物镜 0.5 NA，4 毫米视场），为手术现场提供术中通道。在三名接受开颅手术切除肿瘤的患者中进行的实验表明，FLIM 衍生的参数允许区分肿瘤与正常皮层。例如，在对应于 NADH/NADPH 荧光的 460±25nm 波长带发射处，GBM 表现出较弱的荧光强度（少 35%，p 值<0.05）和较长的寿命 τGBM-Amean=1.59±0.24 ns，而正常皮层 τNC-Amean=1.28±0.04 ns（p 值<0.005）。目前的结果表明，FLIM 有潜力作为脑肿瘤图像引导手术的工具。
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