Feeks James A, Hunter Jennifer J
Center for Visual Science, University of Rochester, Rochester, NY 14627, USA.
The Institute of Optics, University of Rochester, Rochester, NY 14620, USA.
Biomed Opt Express. 2017 Apr 17;8(5):2483-2495. doi: 10.1364/BOE.8.002483. eCollection 2017 May 1.
cellular scale fluorescence lifetime imaging of the mouse retina has the potential to be a sensitive marker of retinal cell health. In this study, we demonstrate fluorescence lifetime imaging of extrinsic fluorophores using adaptive optics fluorescence lifetime imaging ophthalmoscopy (AOFLIO). We recorded AOFLIO images of inner retinal cells labeled with enhanced green fluorescent protein (EGFP) and capillaries labeled with fluorescein. We demonstrate that AOFLIO can be used to differentiate spectrally overlapping fluorophores in the retina. With further refinements, AOFLIO could be used to assess retinal health in early stages of degeneration by utilizing lifetime-based sensors or even fluorophores native to the retina.
小鼠视网膜的细胞尺度荧光寿命成像有潜力成为视网膜细胞健康状况的敏感标志物。在本研究中,我们利用自适应光学荧光寿命成像检眼镜(AOFLIO)展示了外源性荧光团的荧光寿命成像。我们记录了用增强型绿色荧光蛋白(EGFP)标记的视网膜内层细胞以及用荧光素标记的毛细血管的AOFLIO图像。我们证明AOFLIO可用于区分视网膜中光谱重叠的荧光团。通过进一步改进,AOFLIO可利用基于寿命的传感器甚至视网膜内源性荧光团,在视网膜退化的早期阶段评估视网膜健康状况。