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ISG15 促进细胞对登革热和西尼罗河病毒感染的抗病毒反应体外。

ISG15 facilitates cellular antiviral response to dengue and west nile virus infection in vitro.

机构信息

Institute of Biology and Medical Sciences, Jiangsu Key Laboratory of Infection and Immunity, Soochow University, Suzhou 215123, P.R. China.

出版信息

Virol J. 2011 Oct 13;8:468. doi: 10.1186/1743-422X-8-468.

DOI:10.1186/1743-422X-8-468
PMID:21992229
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3215395/
Abstract

BACKGROUND

Dengue virus (DENV) and West Nile virus (WNV), close siblings of the Flaviviridae family, are the causative agents of Dengue hemorraghic shock or West Nile meningoencephalitis respectively. Vaccines against these two flaviviruses are currently unavailable. Interferon- Stimulated Gene 15 (ISG15), encoding an ubiquitin-like protein, is significantly induced by type I interferons or viral infections. Its roles in viral infections, however, vary with viruses, being either anti- or pro-viral. The exact roles of ISG15 in DENV and WNV infections remain unknown. In the current study, we evaluated the relevancies of ISG15 to DENV and WNV infection of a mouse macrophage cell line RAW264.7.

FINDINGS

Quantitative PCR showed that mouse Isg15 was dramatically induced in DENV or WNV- infected RAW264.7 cells compared with non-infected cells. Isg15 and two other Jak-Stat related genes, Socs1 and Socs3, were silenced using siRNA mediated RNA interference. The intracellular DENV and WNV loads, as determined by quantitative PCR, were significantly higher in Isg15 silenced cells than control cells. The expression levels of interferon beta 1 (Ifnb1) were increased significantly in Isg15, Socs1 or Socs3 siRNA treated cells. Further investigation indicated that protein modification by ISG15, so called ISGylation, was significantly enhanced in DENV-infected cells compared to that in non-infected cells.

CONCLUSIONS

These findings suggest that ISG15 plays an anti-DENV/WNV function via protein ISGylation.

摘要

背景

登革热病毒（DENV）和西尼罗河病毒（WNV），黄病毒科的近亲，分别是登革出血热或西尼罗河脑膜脑炎的病原体。目前还没有针对这两种黄病毒的疫苗。干扰素刺激基因 15（ISG15），编码一种泛素样蛋白，可被 I 型干扰素或病毒感染显著诱导。然而，其在病毒感染中的作用因病毒而异，可能是抗病毒或促病毒。ISG15 在 DENV 和 WNV 感染中的确切作用仍不清楚。在本研究中，我们评估了 ISG15 与 DENV 和 WNV 感染小鼠巨噬细胞 RAW264.7 细胞的相关性。

结果

定量 PCR 显示，与未感染细胞相比，DENV 或 WNV 感染的 RAW264.7 细胞中鼠 Isg15 明显被诱导。用 siRNA 介导的 RNA 干扰沉默 Isg15 和另外两个 Jak-Stat 相关基因 Socs1 和 Socs3。通过定量 PCR 测定的细胞内 DENV 和 WNV 载量在 Isg15 沉默细胞中明显高于对照细胞。Isg15、Socs1 或 Socs3 siRNA 处理细胞中干扰素 beta 1（Ifnb1）的表达水平显著增加。进一步的研究表明，与未感染细胞相比，ISG15 蛋白修饰（即 ISGylation）在 DENV 感染细胞中显著增强。

结论

这些发现表明，ISG15 通过蛋白 ISGylation 发挥抗 DENV/WNV 功能。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4dec/3215395/44a1586ed37f/1743-422X-8-468-4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4dec/3215395/2f790381b56d/1743-422X-8-468-1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4dec/3215395/e9d7c422b74e/1743-422X-8-468-2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4dec/3215395/3832ef880b1f/1743-422X-8-468-3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4dec/3215395/44a1586ed37f/1743-422X-8-468-4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4dec/3215395/2f790381b56d/1743-422X-8-468-1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4dec/3215395/e9d7c422b74e/1743-422X-8-468-2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4dec/3215395/3832ef880b1f/1743-422X-8-468-3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4dec/3215395/44a1586ed37f/1743-422X-8-468-4.jpg

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2
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Subcell Biochem. 2010;54:228-37. doi: 10.1007/978-1-4419-6676-6_18.
3
Immune response to dengue virus and prospects for a vaccine.登革热病毒的免疫反应和疫苗前景。
Analyzing the Molecular Signature Genes and Pathways of Dengue Fever, Dengue Hemorrhagic Fever and Dengue Shock Syndrome Caused by Dengue Virus in India.
分析印度登革病毒引起的登革热、登革出血热和登革休克综合征的分子特征基因及通路。
Mol Biotechnol. 2025 Feb 22. doi: 10.1007/s12033-025-01407-7.
4
Harnessing defective interfering particles and lipid nanoparticles for effective delivery of an anti-dengue virus RNA therapy.利用缺陷干扰颗粒和脂质纳米颗粒实现抗登革病毒RNA疗法的有效递送。
Mol Ther Nucleic Acids. 2024 Dec 12;36(1):102424. doi: 10.1016/j.omtn.2024.102424. eCollection 2025 Mar 11.
5
RIPK3 promotes brain region-specific interferon signaling and restriction of tick-borne flavivirus infection.RIPK3 促进大脑区域特异性干扰素信号传导和限制蜱传黄病毒感染。
PLoS Pathog. 2023 Nov 27;19(11):e1011813. doi: 10.1371/journal.ppat.1011813. eCollection 2023 Nov.
6
Type I/type III IFN and related factors regulate JEV infection and BBB endothelial integrity.I 型/III 型 IFN 及相关因子调控 JEV 感染和 BBB 内皮完整性。
J Neuroinflammation. 2023 Sep 27;20(1):216. doi: 10.1186/s12974-023-02891-x.
7
RIPK3 promotes hantaviral replication by restricting JAK-STAT signaling without triggering necroptosis.RIPK3 通过限制 JAK-STAT 信号而不触发坏死性凋亡来促进汉坦病毒复制。
Virol Sin. 2023 Oct;38(5):741-754. doi: 10.1016/j.virs.2023.08.006. Epub 2023 Aug 25.
8
ISG15: its roles in SARS-CoV-2 and other viral infections.ISG15：在 SARS-CoV-2 及其他病毒感染中的作用。
Trends Microbiol. 2023 Dec;31(12):1262-1275. doi: 10.1016/j.tim.2023.07.006. Epub 2023 Aug 10.
9
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J Vet Sci. 2023 Jul;24(4):e53. doi: 10.4142/jvs.23017.
10
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Viruses. 2022 Nov 21;14(11):2575. doi: 10.3390/v14112575.
Annu Rev Immunol. 2011;29:587-619. doi: 10.1146/annurev-immunol-031210-101315.
4
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J Interferon Cytokine Res. 2011 Jan;31(1):119-30. doi: 10.1089/jir.2010.0110. Epub 2010 Dec 29.
5
Emerging role of ISG15 in antiviral immunity.ISG15 在抗病毒免疫中的新兴作用。
Cell. 2010 Oct 15;143(2):187-90. doi: 10.1016/j.cell.2010.09.033.
6
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J Immunol. 2010 Oct 1;185(7):4311-8. doi: 10.4049/jimmunol.1000098. Epub 2010 Sep 1.
7
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Mol Cell. 2010 Jun 11;38(5):722-32. doi: 10.1016/j.molcel.2010.05.002.
8
ISG15 and immune diseases.ISG15与免疫疾病。
Biochim Biophys Acta. 2010 May;1802(5):485-96. doi: 10.1016/j.bbadis.2010.02.006. Epub 2010 Feb 12.
9
ISG15 conjugation system targets the viral NS1 protein in influenza A virus-infected cells.ISG15 缀合系统靶向甲型流感病毒感染细胞中的病毒 NS1 蛋白。
Proc Natl Acad Sci U S A. 2010 Feb 2;107(5):2253-8. doi: 10.1073/pnas.0909144107. Epub 2010 Jan 19.
10
ISG15 over-expression inhibits replication of the Japanese encephalitis virus in human medulloblastoma cells.ISG15 的过表达抑制了乙型脑炎病毒在人髓母细胞瘤细胞中的复制。
Antiviral Res. 2010 Mar;85(3):504-11. doi: 10.1016/j.antiviral.2009.12.007. Epub 2009 Dec 24.