Bio Laboratory, Samsung Advanced Institute of Technology, Samsung Electronics Co., Ltd., Yongin-si, Gyeonggi-do, Republic of Korea.
Anal Chem. 2011 Nov 15;83(22):8629-35. doi: 10.1021/ac2020849. Epub 2011 Oct 26.
We present a rapid and sensitive surface acoustic wave (SAW) immunosensor that utilizes gold staining as a signal enhancement method. A sandwich immunoassay was performed on sensing area of the SAW sensor, which could specifically capture and detect cardiac markers (cardiac troponin I (cTnI), creatine kinase (CK)-MB, and myoglobin). The analytes in human serum were captured on gold nanoparticles (AuNPs) that were conjugated in advance with detection antibodies. Introduction of these complexes to the capture antibody-immobilized sensor surface resulted in a classic AuNP-based sandwich immunoassay format that has been used for signal amplification. In order to achieve further signal enhancement, a gold staining method was performed, which demonstrated that it is possible to obtain gold staining-mediated signal augmentation on a mass-sensitive device. The sensor response due to gold staining varied as a function of cardiac marker concentration. We also investigated effects of increasing operating frequency on sensor responses. Results showed that detection limit of the SAW sensor could be further improved by increasing the operating frequency.
我们提出了一种快速灵敏的表面声波（SAW）免疫传感器，该传感器利用金染色作为信号增强方法。夹心免疫分析在 SAW 传感器的传感区域进行，该区域可以特异性地捕获和检测心脏标志物（心肌肌钙蛋白 I（cTnI）、肌酸激酶（CK）-MB 和肌红蛋白）。在人血清中的分析物被预先与检测抗体结合的金纳米粒子（AuNPs）捕获。将这些复合物引入到捕获抗体固定化传感器表面导致了经典的基于 AuNP 的夹心免疫分析形式，该形式已用于信号放大。为了实现进一步的信号增强，进行了金染色方法，该方法表明在质量敏感的器件上进行金染色介导的信号增强是可能的。由于金染色引起的传感器响应随心脏标志物浓度的变化而变化。我们还研究了增加工作频率对传感器响应的影响。结果表明，通过增加工作频率可以进一步提高 SAW 传感器的检测限。
