• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

使用具有高灵敏度和可重复性的表面声波传感器阵列同时检测从癌细胞中分离的外泌体微小RNA。

Simultaneous Detection of Exosomal microRNAs Isolated from Cancer Cells Using Surface Acoustic Wave Sensor Array with High Sensitivity and Reproducibility.

作者信息

Han Su Bin, Lee Soo Suk

机构信息

Department of Pharmaceutical Engineering, Soonchunhyang University, 22 Soonchunhyang-ro, Shinchang-myeon, Asan-si 31538, Chungcheongnam-do, Republic of Korea.

出版信息

Micromachines (Basel). 2024 Feb 7;15(2):249. doi: 10.3390/mi15020249.

DOI:10.3390/mi15020249
PMID:38398977
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10892992/
Abstract

We present a surface acoustic wave (SAW) sensor array for microRNA (miRNA) detection that utilizes photocatalytic silver staining on titanium dioxide (TiO) nanoparticles as a signal enhancement technique for high sensitivity with an internal reference sensor for high reproducibility. A sandwich hybridization was performed on working sensors of the SAW sensor array that could simultaneously capture and detect three miRNAs (miRNA-21, miRNA-106b, and miRNA-155) known to be upregulated in cancer. Sensor responses due to signal amplification varied depending on the concentration of synthetic miRNAs. It was confirmed that normalization (a ratio of working sensor response to reference sensor response) screened out background interferences by manipulating data and minimized non-uniformity in the photocatalytic silver staining step by suppressing disturbances to both working sensor signal and reference sensor signal. Finally, we were able to successfully detect target miRNAs in cancer cell-derived exosomal miRNAs with performance comparable to the detection of synthetic miRNAs.

摘要

我们展示了一种用于检测微小RNA（miRNA）的表面声波（SAW）传感器阵列，该阵列利用二氧化钛（TiO₂）纳米颗粒上的光催化银染色作为信号增强技术以实现高灵敏度，并采用内部参考传感器以实现高重现性。在SAW传感器阵列的工作传感器上进行了夹心杂交，该阵列能够同时捕获和检测三种已知在癌症中上调的miRNA（miRNA - 21、miRNA - 106b和miRNA - 155）。由于信号放大导致的传感器响应因合成miRNA的浓度而异。证实归一化（工作传感器响应与参考传感器响应的比率）通过处理数据筛选出背景干扰，并通过抑制对工作传感器信号和参考传感器信号的干扰，使光催化银染色步骤中的不均匀性最小化。最后，我们能够成功检测癌细胞衍生的外泌体miRNA中的靶标miRNA，其性能与合成miRNA的检测相当。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6dcd/10892992/9feecf86e2cf/micromachines-15-00249-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6dcd/10892992/3de1630bdf6e/micromachines-15-00249-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6dcd/10892992/e05bbc3432e0/micromachines-15-00249-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6dcd/10892992/1bf16a6bbea5/micromachines-15-00249-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6dcd/10892992/a7362e131615/micromachines-15-00249-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6dcd/10892992/106aa960b689/micromachines-15-00249-g005a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6dcd/10892992/2a997c1acc6a/micromachines-15-00249-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6dcd/10892992/9feecf86e2cf/micromachines-15-00249-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6dcd/10892992/3de1630bdf6e/micromachines-15-00249-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6dcd/10892992/e05bbc3432e0/micromachines-15-00249-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6dcd/10892992/1bf16a6bbea5/micromachines-15-00249-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6dcd/10892992/a7362e131615/micromachines-15-00249-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6dcd/10892992/106aa960b689/micromachines-15-00249-g005a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6dcd/10892992/2a997c1acc6a/micromachines-15-00249-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6dcd/10892992/9feecf86e2cf/micromachines-15-00249-g007.jpg

相似文献

1
Simultaneous Detection of Exosomal microRNAs Isolated from Cancer Cells Using Surface Acoustic Wave Sensor Array with High Sensitivity and Reproducibility.使用具有高灵敏度和可重复性的表面声波传感器阵列同时检测从癌细胞中分离的外泌体微小RNA。
Micromachines (Basel). 2024 Feb 7;15(2):249. doi: 10.3390/mi15020249.
2
Isolation and Characterization of Exosomes from Cancer Cells Using Antibody-Functionalized Paddle Screw-Type Devices and Detection of Exosomal miRNA Using Piezoelectric Biosensor.使用抗体功能化桨叶螺旋式装置从癌细胞中分离和表征外泌体，并使用压电生物传感器检测外泌体 miRNA。
Sensors (Basel). 2024 Aug 21;24(16):5399. doi: 10.3390/s24165399.
3
Sensitive and simultaneous detection of cardiac markers in human serum using surface acoustic wave immunosensor.基于声表面波免疫传感器的人血清中心脏标志物的灵敏和同步检测。
Anal Chem. 2011 Nov 15;83(22):8629-35. doi: 10.1021/ac2020849. Epub 2011 Oct 26.
4
A QCM immunosensor employing signal amplification strategies by enlarging the size of nanoparticles using gold or silver staining.一种采用金或银染色放大纳米粒子尺寸的信号放大策略的 QCM 免疫传感器。
Analyst. 2022 Dec 5;147(24):5725-5731. doi: 10.1039/d2an01726a.
5
Quantitative and Specific Detection of Exosomal miRNAs for Accurate Diagnosis of Breast Cancer Using a Surface-Enhanced Raman Scattering Sensor Based on Plasmonic Head-Flocked Gold Nanopillars.基于等离子体聚集金纳米棒的表面增强拉曼散射传感器用于外泌体 miRNA 的定量和特异性检测，实现乳腺癌的准确诊断。
Small. 2019 Apr;15(17):e1804968. doi: 10.1002/smll.201804968. Epub 2019 Mar 4.
6
Quartz crystal microbalance cardiac Troponin I immunosensors employing signal amplification with TiO nanoparticle photocatalyst.采用TiO纳米颗粒光催化剂进行信号放大的石英晶体微天平心肌肌钙蛋白I免疫传感器。
Talanta. 2021 Jun 1;228:122233. doi: 10.1016/j.talanta.2021.122233. Epub 2021 Feb 19.
7
Quantitative detection of exosomal microRNA extracted from human blood based on surface-enhanced Raman scattering.基于表面增强拉曼散射的人血外泌体 microRNA 的定量检测。
Biosens Bioelectron. 2018 Mar 15;101:167-173. doi: 10.1016/j.bios.2017.08.062. Epub 2017 Oct 11.
8
Comparative Study of Leak Detection in PVC Water Pipes Using Ceramic, Polymer, and Surface Acoustic Wave Sensors.使用陶瓷、聚合物和表面声波传感器对聚氯乙烯（PVC）水管泄漏检测的比较研究。
Sensors (Basel). 2023 Sep 7;23(18):7717. doi: 10.3390/s23187717.
9
An ultrasensitive SERS sensor for simultaneous detection of multiple cancer-related miRNAs.一种用于同时检测多种癌症相关 miRNA 的超灵敏 SERS 传感器。
Nanoscale. 2016 Oct 6;8(39):17365-17373. doi: 10.1039/c6nr05504d.
10
A portable system for isothermal amplification and detection of exosomal microRNAs.一种用于外泌体 microRNAs 的等温扩增和检测的便携式系统。
Biosens Bioelectron. 2022 Jan 15;196:113707. doi: 10.1016/j.bios.2021.113707. Epub 2021 Oct 14.

引用本文的文献

1
Exosomal miRNAs in pancreatitis: Mechanisms and potential applications (Review).胰腺炎中的外泌体微小RNA：机制与潜在应用（综述）
Mol Med Rep. 2025 Aug;32(2). doi: 10.3892/mmr.2025.13575. Epub 2025 May 26.
2
A Versatile SAW Sensor-Based Modular and Portable Platform for a Multi-Sensor Device.一种基于声表面波传感器的多功能模块化便携式多传感器设备平台。
Micromachines (Basel). 2025 Jan 31;16(2):170. doi: 10.3390/mi16020170.
3
Recent Advances in SAW Sensors for Detection of Cancer Biomarkers.用于检测癌症生物标志物的声表面波传感器的最新进展

本文引用的文献

1
Nucleic Acid Molecular Systems for In Vitro Detection of Biomolecules.用于生物分子体外检测的核酸分子系统
ACS Mater Au. 2022 Nov 22;3(2):83-87. doi: 10.1021/acsmaterialsau.2c00056. eCollection 2023 Mar 8.
2
Surface Plasmon Resonance (SPR) Sensor for Cancer Biomarker Detection.用于癌症生物标志物检测的表面等离子体共振 (SPR) 传感器。
Biosensors (Basel). 2023 Mar 17;13(3):396. doi: 10.3390/bios13030396.
3
A Surface Acoustic Wave (SAW)-Based Lab-on-Chip for the Detection of Active α-Glycosidase.基于表面声波（SAW）的芯片实验室用于检测活性 α-糖苷酶。
Biosensors (Basel). 2025 Feb 5;15(2):88. doi: 10.3390/bios15020088.
4
Isolation and Characterization of Exosomes from Cancer Cells Using Antibody-Functionalized Paddle Screw-Type Devices and Detection of Exosomal miRNA Using Piezoelectric Biosensor.使用抗体功能化桨叶螺旋式装置从癌细胞中分离和表征外泌体，并使用压电生物传感器检测外泌体 miRNA。
Sensors (Basel). 2024 Aug 21;24(16):5399. doi: 10.3390/s24165399.
Biosensors (Basel). 2022 Nov 11;12(11):1010. doi: 10.3390/bios12111010.
4
Graphene oxide-Au nano particle coated quartz crystal microbalance biosensor for the real time analysis of carcinoembryonic antigen.用于癌胚抗原实时分析的氧化石墨烯-金纳米颗粒包覆石英晶体微天平生物传感器
RSC Adv. 2020 Jan 24;10(7):4118-4128. doi: 10.1039/c9ra09963h. eCollection 2020 Jan 22.
5
An aptamer-based shear horizontal surface acoustic wave biosensor with a CVD-grown single-layered graphene film for high-sensitivity detection of a label-free endotoxin.一种基于适配体的水平剪切表面声波生物传感器，带有化学气相沉积生长的单层石墨烯薄膜，用于无标记内毒素的高灵敏度检测。
Microsyst Nanoeng. 2020 Feb 10;6:4. doi: 10.1038/s41378-019-0118-6. eCollection 2020.
6
Quartz crystal microbalance cardiac Troponin I immunosensors employing signal amplification with TiO nanoparticle photocatalyst.采用TiO纳米颗粒光催化剂进行信号放大的石英晶体微天平心肌肌钙蛋白I免疫传感器。
Talanta. 2021 Jun 1;228:122233. doi: 10.1016/j.talanta.2021.122233. Epub 2021 Feb 19.
7
Label-Free MicroRNA Optical Biosensors.无标记微小RNA光学生物传感器
Nanomaterials (Basel). 2019 Nov 6;9(11):1573. doi: 10.3390/nano9111573.
8
QCM sensing of miR-21 by formation of microRNA-DNA hybrid duplexes and intercalation on surface-functionalized pyrene.基于微 RNA-DNA 杂合双链体形成和表面功能化芘上的嵌入作用的 QCM 检测 miR-21
Analyst. 2019 Nov 18;144(23):6936-6943. doi: 10.1039/c9an01645g.
9
Amperometric Biosensing of miRNA-21 in Serum and Cancer Cells at Nanostructured Platforms Using Anti-DNA-RNA Hybrid Antibodies.使用抗DNA-RNA杂交抗体在纳米结构平台上对血清和癌细胞中的miRNA-21进行安培生物传感。
ACS Omega. 2018 Aug 9;3(8):8923-8931. doi: 10.1021/acsomega.8b00986. eCollection 2018 Aug 31.
10
In situ monitoring of cytoplasmic precursor and mature microRNA using gold nanoparticle and graphene oxide composite probes.利用金纳米粒子和氧化石墨烯复合探针原位监测细胞质前体和成熟 microRNA。
Anal Chim Acta. 2018 Aug 27;1021:129-139. doi: 10.1016/j.aca.2018.03.010. Epub 2018 Mar 19.