Department of Medical Microbiology and Immunology, University of Wisconsin School of Medicine and Public Health, Madison, WI 53706-1521, USA.
Mol Microbiol. 2011 Nov;82(4):894-903. doi: 10.1111/j.1365-2958.2011.07858.x. Epub 2011 Oct 12.
To successfully colonize and persist within a host niche, bacteria must properly regulate their gene expression profiles. The marine bacterium Vibrio fischeri establishes a mutualistic symbiosis within the light organ of the Hawaiian squid, Euprymna scolopes. Here, we show that the repressor NagC of V. fischeri directly regulates several chitin- and N-acetyl-D-glucosamine-utilization genes that are co-regulated during productive symbiosis. We also demonstrate that repression by NagC is relieved in the presence of N-acetyl-D-glucosamine-6-phosphate, the intracellular form of N-acetyl-D-glucosamine. We find that gene repression by NagC is critical for efficient colonization of E. scolopes. Further, our study shows that NagC regulates genes that affect the normal dynamics of host colonization.
为了成功地在宿主小生境中定殖和生存，细菌必须正确调节其基因表达谱。海洋细菌 Vibrio fischeri 在夏威夷鱿鱼 Euprymna scolopes 的发光器官中建立了互利共生关系。在这里，我们表明 V. fischeri 的抑制剂 NagC 直接调节几种几丁质和 N-乙酰-D-葡萄糖胺利用基因，这些基因在有生产力的共生过程中受到共同调节。我们还证明，在存在 N-乙酰-D-葡萄糖胺-6-磷酸（N-乙酰-D-葡萄糖胺的细胞内形式）的情况下，NagC 的抑制作用得到缓解。我们发现，NagC 对 E. scolopes 的有效定殖至关重要。此外，我们的研究表明，NagC 调节影响宿主定殖正常动态的基因。
