Department of Pharmacology, All India Institute of Medical Sciences, New Delhi 110029, India.
Epilepsy Res. 2012 Feb;98(2-3):157-65. doi: 10.1016/j.eplepsyres.2011.09.006. Epub 2011 Oct 12.
In Ayurveda, Anacyclus pyrethrum has been used as a brain tonic. The present study evaluates the effect of hydroalcoholic extract of A. pyrethrum (HEAP) root against seizures, seizure-induced oxidative stress and cognitive impairment in experimental models of seizures. Male Wistar rats were used in the study. HEAP was administered in doses of 50, 100, 250, 500 in pentylenetetrazole (PTZ) model and 250, 500 and 1000 mg/kg in maximal electroshock (MES) model. Myoclonic jerk latency and generalized tonic clonic seizures (GTCS) were noted in PTZ whereas occurrence of tonic hind limb extension (THLE) was observed in MES seizures. Cognitive deficit was assessed using elevated plus maze and passive avoidance tests. Whole brain reduced glutathione, malondialdehyde levels and cholinesterase activity were measured. HEAP showed 50, 66.7, 83.3 and 100% protection at 50,100, 250 and 500 mg/kg, respectively against GTCS in PTZ induced seizures. In MES induced seizures, HEAP produced 16.7, 33.3 and 50% protection against THLE at 250, 500 and 1000 mg/kg, respectively. HEAP administration significantly prevented seizure induced oxidative stress and cognitive impairment in a dose-dependent manner. HEAP also normalized the decrease in cholinesterase activity caused by seizures. Thus, HEAP showed protective effect against seizures, seizure-induced oxidative stress and cognitive impairment in rats.
在阿育吠陀医学中，春黄菊已被用作大脑滋补品。本研究评估了春黄菊根的水醇提取物（HEAP）根对实验性癫痫模型中的癫痫发作、癫痫发作引起的氧化应激和认知障碍的影响。雄性 Wistar 大鼠用于本研究。HEAP 以 50、100、250 和 500 剂量在戊四氮（PTZ）模型中给予，以 250、500 和 1000 mg/kg 在最大电休克（MES）模型中给予。在 PTZ 中观察到肌阵挛性抽搐潜伏期和全身性强直阵挛性发作（GTCS），而在 MES 癫痫发作中观察到强直性后肢伸展（THLE）。使用高架十字迷宫和被动回避试验评估认知缺陷。测量全脑还原型谷胱甘肽、丙二醛水平和胆碱酯酶活性。HEAP 在 50、100、250 和 500 mg/kg 时分别对 GTCS 提供 50、66.7、83.3 和 100%的保护，对 PTZ 诱导的癫痫发作有保护作用。在 MES 诱导的癫痫发作中，HEAP 在 250、500 和 1000 mg/kg 时分别对 THLE 产生 16.7、33.3 和 50%的保护作用。HEAP 给药以剂量依赖性方式显著防止癫痫发作引起的氧化应激和认知障碍。HEAP 还使癫痫发作引起的胆碱酯酶活性降低正常化。因此，HEAP 对大鼠的癫痫发作、癫痫发作引起的氧化应激和认知障碍表现出保护作用。
