Department of Urology, The Second Xiangya Hospital of Central South University, Changsha, Hunan 410011, PR China.
Mol Med Rep. 2012 Jan;5(1):260-5. doi: 10.3892/mmr.2011.621. Epub 2011 Oct 11.
Numerous microRNAs (miRNAs) play crucial roles in cancer development. In this study, we report that hsa-miR-96 is expressed at higher levels in human bladder urothelial carcinomas compared to normal tissues. We found that hsa-miR-96 increased invasion and differentiation of human bladder T24 cells and promoted their growth. Down‑regulation of hsa-miR-96 significantly affected the phenotype of bladder cancer T24 cells. The mRNA and protein levels of insulin receptor substrate 1 (IRS1) and MAP4K1 were significantly reduced in cells transfected with the hsa-miR-96 inhibitor when compared with levels in cells transfected with the empty plasmid vector or the negative control miRNA inhibitor. Altogether, these results suggest that hsa-miR-96 may affect the growth of bladder cancer cells by up-regulating IRS1 and MAP4K1 levels, functioning as a promising diagnostic marker in human bladder urothelial carcinomas.
许多 microRNAs（miRNAs）在癌症发展中发挥着关键作用。在这项研究中，我们报告 hsa-miR-96 在人膀胱尿路上皮癌组织中的表达水平高于正常组织。我们发现 hsa-miR-96 可增加人膀胱 T24 细胞的侵袭和分化，并促进其生长。下调 hsa-miR-96 可显著影响膀胱癌 T24 细胞的表型。与空载质粒载体或阴性对照 miRNA 抑制剂转染的细胞相比，hsa-miR-96 抑制剂转染的细胞中胰岛素受体底物 1（IRS1）和 MAP4K1 的 mRNA 和蛋白水平显著降低。总之，这些结果表明 hsa-miR-96 可能通过上调 IRS1 和 MAP4K1 水平影响膀胱癌细胞的生长，可作为人膀胱尿路上皮癌有前途的诊断标志物。
