Sluiter W, Hulsing-Hesselink E, Elzenga-Claasen I, Van Furth R
J Immunol Methods. 1985 Jan 21;76(1):135-43. doi: 10.1016/0022-1759(85)90486-7.
Collection of small amounts of blood from the orbital sinus was found to be a satisfactory method for repeated sampling in mice, which means that these animals can be selected for further study on the basis of the leukocyte count. In biomedical research it is often necessary to have detailed information about the effect of injected material on the numerical course of circulating leukocytes. However, the present study has shown that 2 stress-producing procedures on 1 day disturb the steady state, and that this disturbance is expressed in changes in the number of leukocytes. Such stress could be avoided by alteration of the experimental design to include only 1 stressful situation each day. When blood was sampled in the orbital sinus on 1 day to determine the animals' condition and on the next day only the tail was punctured as sham injection, the number of blood leukocytes remained constant throughout the observation period. Comparative studies on the numbers of monocytes, lymphocytes, and granulocytes in blood from the tail, heart, and orbital sinus showed a systematic difference in the mean numbers of certain types of leukocytes. Statistically significant differences were found between the mean numbers of monocytes, lymphocytes, and granulocytes in orbital blood from normal mice of 5 specific pathogen-free strains, i.e., Cpb:SE (Swiss), CBA/Cpb, BALB/cCpb, C3H/Rij, and DBA/2Rij.
从小鼠眶窦采集少量血液被发现是一种令人满意的重复采样方法，这意味着可以根据白细胞计数选择这些动物进行进一步研究。在生物医学研究中，通常需要详细了解注射物质对循环白细胞数量变化过程的影响。然而，本研究表明，一天内进行两次产生应激的操作会干扰稳态，并且这种干扰表现为白细胞数量的变化。通过改变实验设计，每天只包含一种应激情况，可以避免这种应激。当在一天从眶窦采集血液以确定动物状况，而在第二天仅穿刺尾巴作为假注射时，在整个观察期内血液白细胞数量保持恒定。对来自尾巴、心脏和眶窦的血液中的单核细胞、淋巴细胞和粒细胞数量进行的比较研究表明，某些类型白细胞的平均数量存在系统性差异。在5种特定无特定病原体品系的正常小鼠，即Cpb:SE（瑞士小鼠）、CBA/Cpb、BALB/cCpb、C3H/Rij和DBA/2Rij的眶血中，单核细胞、淋巴细胞和粒细胞的平均数量之间发现了具有统计学意义的差异。
