含中药血清对 ox-LDL 诱导的内皮细胞炎症因子表达的影响。
Effect of chinese herbal drug-containing serum for activating-blood and dispelling-toxin on ox-LDL-induced inflammatory factors' expression in endothelial cells.
机构信息
Department of Cardiovascular Diseases, Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing, 100091, China.
出版信息
Chin J Integr Med. 2012 Jan;18(1):30-3. doi: 10.1007/s11655-011-0849-1. Epub 2011 Oct 12.
OBJECTIVE
To investigate the effects of drug-containing serum of Chinese herbal compound, Xiongshao Capsule (, XS, for activating-blood) and Huanglian Capsule (, HL, for dispellingtoxin) on the oxidized low-density lipoprotein (ox-LDL)-induced inflammatory factors in human umbilical vein endothelial cells (HUVECs).
METHODS
Thirty-two rats were randomly divided into four groups: the blank control group treated with distilled water, the positive control group treated with simvastatin (1.8 mg/kg), the test group I treated with Chinese herbal compound of XS (0.135 g/kg), and the test group II treated with Chinese herbal compound of XS (0.135 g/kg) and HL (0.135 g/kg). All the treatments were administered for 7 successive days by gastrogavage. Rats' blood serum was harvested 1 h after the last administration to prepare respective drugcontaining serum. HUVECs were exposed to ox-LDL (100 μg/mL) to induce cell injury model and incubated with corresponding drug-containing serum for 24 h. Untreated HUVECs were set for blank control. Levels of interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), and soluble intercellular adhesion molecule-1 (sICAM-1) in supernatant of cultured HUVECs were determined by enzyme-linked immunosorbent assay (ELISA). HUVEC surface expressions of ICAM-1 and E-selectin were determined by flow cytometry.
RESULTS
Levels of IL-6, TNF-α, and sICAM-1 in the supernatant of HUVECs as well as the cell surface expressions of ICAM-1 and E-selectin significantly increased after 24-h ox-LDL stimulation (P<0.01), while the abnormal elevations, except sICAM-1 in the test group I, were all reduced in the treated groups (the positive control and the two test groups) significantly (P<0.01 or P<0.05). Besides, the effect in the test group II seemed somewhat higher than that in the test group I but with no statistical significance (P>0.05).
CONCLUSION
Drug-containing serum of XS plus HL has a certain inhibitory effect on the vascular endothelial inflammation response induced by ox-LDL.
目的
观察芎芍胶囊（XS）、黄连胶囊（HL）含药血清对氧化型低密度脂蛋白（ox-LDL）诱导的人脐静脉内皮细胞（HUVEC）炎症因子的影响。
方法
32 只大鼠随机分为 4 组，空白对照组给予蒸馏水，阳性对照组给予辛伐他汀（1.8 mg/kg），XS 低、高剂量组分别给予 XS 含药血清（0.135 g/kg）和 XS 加 HL 含药血清（0.135 g/kg），每日 1 次灌胃给药，连续 7 天，末次给药 1 h 后腹主动脉采血制备含药血清。将 HUVEC 与 ox-LDL（100 μg/mL）共同孵育 24 h 建立细胞损伤模型，分别加入相应含药血清培养 24 h，设正常对照组（未加 ox-LDL）。采用酶联免疫吸附法（ELISA）检测细胞上清液中白细胞介素 6（IL-6）、肿瘤坏死因子-α（TNF-α）、可溶性细胞间黏附分子 1（sICAM-1）的含量，流式细胞术检测 HUVEC 表面细胞间黏附分子 1（ICAM-1）、E-选择素的表达。
结果
ox-LDL 作用 24 h 后，HUVEC 上清液中 IL-6、TNF-α、sICAM-1 水平及细胞表面 ICAM-1、E-选择素表达均显著升高（P<0.01）；各给药组上述异常升高均明显降低（P<0.01 或 P<0.05），其中 XS 加 HL 含药血清组 sICAM-1 水平降低更明显，但与 XS 高剂量组比较差异无统计学意义（P>0.05）。
结论
XS 加 HL 含药血清对 ox-LDL 诱导的血管内皮炎症反应具有一定抑制作用。
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