School of Food and Life Science, Inje University, 607 Obang-dong, Gimhae, Gyungnam 621-749, Korea.
Nutr Res Pract. 2011 Aug;5(4):365-9. doi: 10.4162/nrp.2011.5.4.365. Epub 2011 Aug 31.
This study investigated the preventive effect of Saururus chinensis Baill against renal damage induced by a high-fructose diet in rats. The rats (n = 30) were fed either a cornstarch-based (65%), high-fructose (65%), or high-fructose (64.5%) diet with 0.5% S. chinensis Baill extract for 10 weeks. Twenty-four hour urine collections were obtained and the animals were sacrificed after an overnight fast. Serum urea and creatinine and urine albumin were measured using colorimetric methods, and creatinine clearance was determined. In addition, thiobarbituric acid reactive substances (TBARS), reduced glutathione (GSH), and the activity of superoxide dismutase (SOD) in the kidney were determined. Kidney samples were also examined histologically. The fructose-fed rats showed renal dysfunction, indicated by decreased creatinine clearance, increased albumin in the urine, and increased urea and creatinine in the serum. These renal function parameters were comparable to control levels in rats that consumed S. chinensis Baill. Fructose consumption increased renal TBARS and reduced GSH and SOD activity, whereas these levels were near-normal in the rats consuming S. chinensis Baill. The kidneys of fructose-fed rats showed glomerular basement membrane thickening, mesangial matrix expansion, and tubule dilation. These pathological changes were not seen in the rats that consumed S. chinensis Baill. Therefore, S. chinensis Baill effectively alleviated fructose-induced renal damage in these rats, at least partially due to antioxidant activity.
本研究探讨了蛇菰预防高果糖饮食诱导的大鼠肾损伤的作用。30 只大鼠分别给予玉米淀粉基础（65%）、高果糖（65%）或高果糖（64.5%）饮食，同时给予 0.5%蛇菰提取物，共 10 周。收集 24 小时尿液，动物禁食过夜后处死。采用比色法测定血清尿素和肌酐以及尿白蛋白，计算肌酐清除率。此外，还测定了肾组织中超氧化物歧化酶（SOD）的活性。肾组织匀浆测定丙二醛（MDA）、还原型谷胱甘肽（GSH）含量和超氧化物歧化酶（SOD）活性。果糖喂养的大鼠出现肾功能障碍，表现为肌酐清除率降低，尿白蛋白增加，血清尿素和肌酐升高。这些肾功能参数与食用蛇菰的大鼠相比接近正常水平。果糖摄入增加了肾组织 MDA 和降低了 GSH 和 SOD 活性，而食用蛇菰的大鼠这些水平接近正常。果糖喂养的大鼠肾小球基底膜增厚、系膜基质扩张和肾小管扩张。而食用蛇菰的大鼠未出现这些病变。因此，蛇菰可有效缓解果糖诱导的大鼠肾损伤，其作用至少部分归因于抗氧化活性。
