Riley L K, Caffrey C J
Department of Veterinary Pathology, University of Missouri, Columbia 65211.
J Clin Microbiol. 1990 Jun;28(6):1465-8. doi: 10.1128/jcm.28.6.1465-1468.1990.
Enterotoxigenic Escherichia coli (ETEC) strains were readily identified in pure and mixed cultures with nonradioactive, digoxigenin-labeled DNA probes coding for heat-labile (LTI) and heat-stable (STaI, STaII, and STb) enterotoxins. Digoxigenin-labeled ETEC fragments were more sensitive than and exhibited less nonspecific background contamination than biotinylated ETEC probes.
利用编码不耐热(LTI)和耐热(STaI、STaII和STb)肠毒素的非放射性地高辛标记DNA探针,可在纯培养物和混合培养物中轻松鉴定产肠毒素大肠杆菌(ETEC)菌株。与生物素化的ETEC探针相比,地高辛标记的ETEC片段更灵敏,且非特异性背景污染更少。