用非放射性地高辛标记的DNA探针通过菌落杂交法鉴定产肠毒素大肠杆菌
Identification of enterotoxigenic Escherichia coli by colony hybridization with nonradioactive digoxigenin-labeled DNA probes.
作者信息
Riley L K, Caffrey C J
机构信息
Department of Veterinary Pathology, University of Missouri, Columbia 65211.
出版信息
J Clin Microbiol. 1990 Jun;28(6):1465-8. doi: 10.1128/jcm.28.6.1465-1468.1990.
Abstract
Enterotoxigenic Escherichia coli (ETEC) strains were readily identified in pure and mixed cultures with nonradioactive, digoxigenin-labeled DNA probes coding for heat-labile (LTI) and heat-stable (STaI, STaII, and STb) enterotoxins. Digoxigenin-labeled ETEC fragments were more sensitive than and exhibited less nonspecific background contamination than biotinylated ETEC probes.
摘要
利用编码不耐热（LTI）和耐热（STaI、STaII和STb）肠毒素的非放射性地高辛标记DNA探针，可在纯培养物和混合培养物中轻松鉴定产肠毒素大肠杆菌（ETEC）菌株。与生物素化的ETEC探针相比，地高辛标记的ETEC片段更灵敏，且非特异性背景污染更少。
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