Department of Pharmacognosy, Faculty of Pharmacy, Hacettepe University, Ankara, Turkey.
Nat Prod Res. 2012;26(13):1261-4. doi: 10.1080/14786419.2011.578071. Epub 2011 Oct 14.
Chromatographic studies on the EtOAc soluble portion of the MeOH extract of Geranium lasiopus led to the isolation of eight flavonoids (kaempferol (1), quercetin (2), quercetin 3-O-β-glucopyranoside (3), quercetin 3-O-β-galactopyranoside (4), kaempferol 3-O-α-rhamnopyranosyl-(1 → 6)-β-glucopyranoside (5), quercetin 3-O-α-rhamnopyranosyl-(1 → 6)-β-glucopyranoside (6), kaempferol 3-O-α-rhamnopyranosyl-(1 → 2)-β-glucopyranoside (7) and quercetin 3-O-α-rhamnopyranosyl-(1 → 2)-β-glucopyranoside (8)), two simple phenolic compounds (gallic acid (9) and its methyl ester (10)) and a hydrolysable tannin (pusilagin (11)). The structures of the compounds were elucidated by 1- and 2-dimensional NMR techniques ((1)H, (13)C, COSY, HMBC, HMQC) and ESI-TOF-MS spectrometry. Inhibitory effects on H(2)O(2)-induced lipid peroxidation in human red blood cells of the different extracts of G. lasiopus, as well as isolated compounds, were investigated. All tested compounds showed comparable or higher activity than that of ascorbic acid and trolox.
从老鹳草的 MeOH 提取物的 EtOAc 可溶部分的色谱研究中，分离得到了 8 种黄酮类化合物（山柰酚（1）、槲皮素（2）、槲皮素 3-O-β-吡喃葡萄糖苷（3）、槲皮素 3-O-β-半乳糖吡喃糖苷（4）、山柰酚 3-O-α-鼠李吡喃糖基-(1 → 6)-β-吡喃葡萄糖苷（5）、槲皮素 3-O-α-鼠李吡喃糖基-(1 → 6)-β-吡喃葡萄糖苷（6）、山柰酚 3-O-α-鼠李吡喃糖基-(1 → 2)-β-吡喃葡萄糖苷（7）和槲皮素 3-O-α-鼠李吡喃糖基-(1 → 2)-β-吡喃葡萄糖苷（8））、2 种简单酚类化合物（没食子酸（9）及其甲酯（10））和一种可水解鞣质（pusilagin（11））。通过 1D 和 2D NMR 技术（（1）H、（13）C、COSY、HMBC、HMQC）和 ESI-TOF-MS 光谱法阐明了化合物的结构。研究了不同提取物和分离化合物对人红细胞中 H(2)O(2)诱导的脂质过氧化的抑制作用。所有测试的化合物都显示出与抗坏血酸和 Trolox 相当或更高的活性。
