Department of Biotechnology and Food Microbiology, Poznan University of Life Sciences, Poznañ, Poland.
Crit Rev Biotechnol. 2012 Sep;32(3):274-88. doi: 10.3109/07388551.2011.616859. Epub 2011 Oct 13.
Klebsiella spp are one of the best natural producers of 1,3-propanediol (1,3-PD). However, their usage in the biotechnological production of the diol is limited, since the species belong to the second hazard group. Nevertheless, multiple advantageous traits of Klebsiella spp justify the international effort devoted to develop a biotechnological process of 1,3-PD production with these microorganisms. Apart from the process engineering approach aiming at improvement of 1,3-PD production by Klebsiella spp, plethora of metabolic engineering approaches have been reported. Different strategies have been undertaken to genetically improve Klebsiella strains and provide them with the ability to synthesize 1,3-PD more efficiently. These include over-expression of both homologous and heterologous genes of the 1,3-PD synthesis pathway, protein and cofactor engineering, deletion of the genes involved in by-products formation. This review provides an overview of the initial and most recent reports on the metabolic engineering of Klebsiella spp with the aim of improvement of 1,3-PD biosynthesis.
产酸克雷伯氏菌是 1,3-丙二醇（1,3-PD）的最佳天然生产者之一。然而，由于这些物种属于第二危害组，因此它们在该二醇的生物技术生产中的应用受到限制。尽管如此，产酸克雷伯氏菌的多种有利特性证明了国际社会致力于开发使用这些微生物生产 1,3-PD 的生物技术工艺的努力是合理的。除了旨在通过产酸克雷伯氏菌提高 1,3-PD 产量的过程工程方法外，还报道了大量的代谢工程方法。已经采取了不同的策略来遗传改良克雷伯氏菌菌株，并赋予它们更有效地合成 1,3-PD 的能力。这些策略包括过表达 1,3-PD 合成途径的同源和异源基因、蛋白质和辅因子工程、删除参与副产物形成的基因。本文综述了产酸克雷伯氏菌代谢工程的初始和最新报告，旨在提高 1,3-PD 生物合成。
