Translational Medical Sciences, Division of Applied Medicine, School of Medicine and Dentistry, University of Aberdeen, Aberdeen, AB25 2ZD, UK.
Prostaglandins Leukot Essent Fatty Acids. 2011 Dec;85(6):305-10. doi: 10.1016/j.plefa.2011.09.007. Epub 2011 Oct 12.
Omega-3 (n-3) fatty acids inhibit breast and prostate cancer cell growth. We previously showed that N-acylethanolamine derivatives of n-3 (n-3-NAE) are endocannabinoids, which regulate cancer cell proliferation. These n-3-NAE are synthesised in certain cells/tissues, after supplementing with fatty acids, however, no one has assessed whether and to what extent this occurs in cancer cells. We determined levels of endogenous n-3-NAEs in hormone sensitive and insensitive prostate and breast cancer cells and subsequent effects on other endocannabinoids (anandamide and 2-arachidonoylglycerol), before and after supplementing with DHA and EPA fatty acids, using HPLC tandem mass spectrometry. This is the first study reporting that n-3-NAEs are synthesised from their parent n-3 fatty acids in cancer cells, regardless of tumour type, hormone status or the presence of fatty acid amide hydrolase. This could have important implications for the use of n-3 fatty acids as therapeutic agents in breast and prostate cancers expressing cannabinoid receptors.
ω-3（n-3）脂肪酸抑制乳腺癌和前列腺癌细胞生长。我们之前曾表明，n-3（n-3-NAE）的酰基乙醇胺衍生物是内源性大麻素，可调节癌细胞增殖。这些 n-3-NAE 在补充脂肪酸后，在某些细胞/组织中合成，但是，没有人评估过这种情况是否以及在多大程度上发生在癌细胞中。我们使用 HPLC 串联质谱法测定了激素敏感和不敏感的前列腺癌和乳腺癌细胞中内源性 n-3-NAE 的水平，以及在补充 DHA 和 EPA 脂肪酸前后对其他内源性大麻素（花生四烯酸和 2-花生四烯酰甘油）的影响。这是第一项报道 n-3-NAE 可从其亲本 n-3 脂肪酸在癌细胞中合成的研究，无论肿瘤类型、激素状态或脂肪酸酰胺水解酶的存在如何。这对于在表达大麻素受体的乳腺癌和前列腺癌中使用 n-3 脂肪酸作为治疗剂具有重要意义。
