Department of Pathology, University of Veterinary Medicine Hannover, Hannover, Germany.
Vet Microbiol. 2012 Mar 23;155(2-4):420-4. doi: 10.1016/j.vetmic.2011.09.020. Epub 2011 Sep 25.
An equid herpesvirus 5 (EHV-5) infection was detected in lesioned skin from a nine-year-old Holsteiner stallion in the south of Germany. Macroscopically, the animal displayed a non-pruritic, multifocal, pustular dermatitis around both eyes, nostrils and the muzzle, which had been ongoing for one year. Histopathologically, skin lesions were characterized by orthokeratotic to parakeratotic hyperkeratosis, pustular dermatitis, epidermal hyperplasia, apoptotic keratinocytes, a lympho-plasmahistiocytic interface dermatitis with hydropic degeneration of keratinocytes, and perivascular to diffuse, lympho-histiocytic infiltrations. The stratum granulosum and the upper part of the stratum spinosum contained multiple amphophilic, intranuclear inclusion bodies. By in situ hybridization and immunohistochemistry herpesvirus DNA and protein, respectively, were detected within keratinocytes containing inclusion bodies. Sequencing of the PCR-product revealed the presence of EHV-5 DNA. This is the first description of a dermatitis associated with EHV-5 in a horse, indicating that EHV-5 should be considered as an etiology of lymphohistiocytic interface dermatitis with intranuclear inclusion bodies in horses and is similar to herpes-associated erythema multiforme in humans.
在德国南部的一匹 9 岁的荷斯坦种公马的病变皮肤中检测到了一种 equid 疱疹病毒 5（EHV-5）感染。从宏观上看，该动物表现出非瘙痒性、多灶性、脓疱性皮炎，发生在双眼周围、鼻孔和口鼻部，已经持续了一年。组织病理学上，皮肤病变的特征是正角化过度到角化不全性角化过度、脓疱性皮炎、表皮增生、凋亡的角质形成细胞、伴有角质形成细胞水样变性的淋巴浆细胞界面性皮炎，以及血管周围至弥漫性、淋巴浆细胞浸润。颗粒层和棘层的上部含有多个嗜碱性、核内包涵体。原位杂交和免疫组织化学分别检测到含有包涵体的角质形成细胞中存在疱疹病毒 DNA 和蛋白。PCR 产物的测序显示存在 EHV-5 DNA。这是首例在马中与 EHV-5 相关的皮炎描述，表明 EHV-5 应被视为马中具有核内包涵体的淋巴浆细胞界面性皮炎的病因，与人疱疹相关性多形红斑相似。
