Reference and Translation Center for Cardiac Stem Cell Therapy (RTC), Department of Cardiac Surgery, University of Rostock, Rostock, Germany.
PLoS One. 2011 Feb 10;6(2):e15652. doi: 10.1371/journal.pone.0015652.
The possible different therapeutic efficacy of human mesenchymal stem cells (hMSC) derived from umbilical cord blood (CB), adipose tissue (AT) or bone marrow (BM) for the treatment of myocardial infarction (MI) remains unexplored. This study was to assess the regenerative potential of hMSC from different origins and to evaluate the role of CD105 in cardiac regeneration. Male SCID mice underwent LAD-ligation and received the respective cell type (400.000/per animal) intramyocardially. Six weeks post infarction, cardiac catheterization showed significant preservation of left ventricular functions in BM and CD105(+)-CB treated groups compared to CB and nontreated MI group (MI-C). Cell survival analyzed by quantitative real time PCR for human GAPDH and capillary density measured by immunostaining showed consistent results. Furthermore, cardiac remodeling can be significantly attenuated by BM-hMSC compared to MI-C. Under hypoxic conditions in vitro, remarkably increased extracellular acidification and apoptosis has been detected from CB-hMSC compared to BM and CD105 purified CB-derived hMSC. Our findings suggests that hMSC originating from different sources showed a different healing performance in cardiac regeneration and CD105(+) hMSC exhibited a favorable survival pattern in infarcted hearts, which translates into a more robust preservation of cardiac function.
尚未探索源自脐血（CB）、脂肪组织（AT）或骨髓（BM）的人骨髓间充质干细胞（hMSC）在治疗心肌梗死（MI）方面可能存在不同的治疗效果。本研究旨在评估不同来源的 hMSC 的再生潜力，并评估 CD105 在心脏再生中的作用。雄性 SCID 小鼠接受 LAD 结扎，并接受相应的细胞类型（每只动物 400,000 个）心肌内注射。心肌梗死 6 周后，心脏导管检查显示与 CB 和未治疗的 MI 组（MI-C）相比，BM 和 CD105(+)CB 处理组的左心室功能明显得到保存。通过定量实时 PCR 分析人类 GAPDH 和免疫染色测量的毛细血管密度来评估细胞存活，结果一致。此外，与 MI-C 相比，BM-hMSC 可显著减轻心脏重塑。在体外缺氧条件下，与 BM 和 CD105 纯化 CB 来源的 hMSC 相比，从 CB-hMSC 中检测到明显增加的细胞外酸化和细胞凋亡。我们的研究结果表明，源自不同来源的 hMSC 在心脏再生中表现出不同的愈合性能，CD105(+)hMSC 在梗死心脏中表现出有利的存活模式，从而更有效地保存心脏功能。
