Biomolecular Frontiers Research Centre, Macquarie University, Australia.
Department of Chemistry and Biomolecular Sciences, Macquarie University, Australia.
Microbiology (Reading). 2012 Jan;158(Pt 1):58-68. doi: 10.1099/mic.0.054031-0. Epub 2011 Oct 13.
The hypersecreting mutant Trichoderma reesei RUT-C30 (ATCC 56765) is one of the most widely used strains of filamentous fungi for the production of cellulolytic enzymes and recombinant proteins, and for academic research. The strain was obtained after three rounds of random mutagenesis of the wild-type QM6a in a screening program focused on high cellulase production and catabolite derepression. Whereas RUT-C30 achieves outstanding levels of protein secretion and high cellulolytic activity in comparison to the wild-type QM6a, recombinant protein production has been less successful. Here, we bring together and discuss the results from biochemical-, microscopic-, genomic-, transcriptomic-, glycomic- and proteomic-based research on the RUT-C30 strain published over the last 30 years.
高产纤维素酶和重组蛋白的丝状真菌瑞氏木霉突变株 RUT-C30（ATCC 56765）是最广泛使用的菌株之一，也被广泛应用于学术研究。该菌株是在以高纤维素酶产量和分解代谢阻遏为目标的野生型 QM6a 的三轮随机诱变筛选计划中获得的。与野生型 QM6a 相比，RUT-C30 实现了出色的蛋白质分泌水平和高纤维素酶活性，但重组蛋白的产量却不尽如人意。在这里，我们汇集并讨论了过去 30 年来基于生物化学、显微镜、基因组学、转录组学、糖组学和蛋白质组学的关于 RUT-C30 菌株的研究结果。
