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Repair of alkylation damage in Saccharomyces cerevisiae.

作者信息

Goth-Goldstein R, Johnson P L

机构信息

Cell and Molecular Biology Division, Lawrence Berkeley Laboratory, Berkeley, CA 94720.

出版信息

Mol Gen Genet. 1990 May;221(3):353-7. doi: 10.1007/BF00259399.

DOI:10.1007/BF00259399
PMID:2199817
Abstract

Repair of methylated bases in Saccharomyces cerevisiae was measured by two methods: in vitro in cell extracts, and in vivo, by determining the loss of methylated bases from yeast DNA after treatment of stationary cultures with [3H]-N-methyl-N'-nitro-N-nitrosoguanidine. Whereas no repair activity could be detected by the in vitro method, the methylated bases were removed in vivo very efficiently. These contradictory results of in vitro and in vivo repair measurements suggest that either the repair enzymes of yeast are sufficiently different from those of bacteria and mammalian cells that they are not active in the in vitro assay, or that methylated bases are repaired in yeast by a different pathway.

摘要

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本文引用的文献

1
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Nature. 1979 Jul 5;280(5717):76-7. doi: 10.1038/280076a0.
2
A comprehensive quantitative analysis of methylated and ethylated DNA using high pressure liquid chromatography.使用高压液相色谱法对甲基化和乙基化DNA进行全面的定量分析。
Carcinogenesis. 1980 Jul;1(7):595-606. doi: 10.1093/carcin/1.7.595.
3
Stimulation of transfer of methyl groups from O6-methylguanine in DNA to protein by rat liver extracts in response to hepatotoxins.
酿酒酵母中一种烷基碱基DNA糖基化酶基因的克隆及其在大肠杆菌中的表达；该基因是细菌alkA基因的同源物。
EMBO J. 1990 Dec;9(13):4563-8. doi: 10.1002/j.1460-2075.1990.tb07909.x.
4
Primary sequence and biological functions of a Saccharomyces cerevisiae O6-methylguanine/O4-methylthymine DNA repair methyltransferase gene.
EMBO J. 1991 Aug;10(8):2179-86. doi: 10.1002/j.1460-2075.1991.tb07753.x.
大鼠肝脏提取物对肝毒素的反应促进DNA中O6-甲基鸟嘌呤的甲基基团向蛋白质转移。
Carcinogenesis. 1981;2(11):1195-200. doi: 10.1093/carcin/2.11.1195.
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The activity of 3-methyladenine DNA glycosylate in animal tissues in relation to carcinogenesis.动物组织中3-甲基腺嘌呤DNA糖基化酶活性与致癌作用的关系。
Carcinogenesis. 1982;3(7):747-50. doi: 10.1093/carcin/3.7.747.
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J Biol Chem. 1980 Nov 25;255(22):10569-71.
6
Escherichia coli mutants deficient in 3-methyladenine-DNA glycosylase.缺乏3-甲基腺嘌呤-DNA糖基化酶的大肠杆菌突变体。
J Mol Biol. 1980 Jun 15;140(1):101-27. doi: 10.1016/0022-2836(80)90358-7.
7
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