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酿酒酵母中的烷基化诱变：缺乏适应性反应的证据。

Alkylation mutagenesis in Saccharomyces cerevisiae: lack of evidence for an adaptive response.

作者信息

Polakowska R, Perozzi G, Prakash L

机构信息

Department of Radiation Biology and Biophysics, University of Rochester School of Medicine, NY 14642.

出版信息

Curr Genet. 1986;10(9):647-55. doi: 10.1007/BF00410912.

DOI:10.1007/BF00410912
PMID:3329040
Abstract

We have found no evidence for an adaptive response for either lethality or mutagenesis following treatment of Saccharomyces cerevisiae with N-methyl-N'-nitro-N-nitrosoguanidine (MNNG). The rad6 and rad52 mutants of S. cerevisiae are highly defective in MNNG and ethyl methanesulfonate induced mutagenesis of both stationary and exponential phase cells. These and other observations indicate that the mechanisms of repair of alkylation damage and mutagenesis differ markedly between S. cerevisiae and Escherichia coli.

摘要

在用N-甲基-N'-硝基-N-亚硝基胍（MNNG）处理酿酒酵母后，我们没有发现其对致死性或诱变产生适应性反应的证据。酿酒酵母的rad6和rad52突变体在MNNG和甲磺酸乙酯诱导的静止期和指数生长期细胞诱变方面存在高度缺陷。这些以及其他观察结果表明，酿酒酵母和大肠杆菌在烷基化损伤修复和诱变机制上存在显著差异。

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Alkylation mutagenesis in Saccharomyces cerevisiae: lack of evidence for an adaptive response.酿酒酵母中的烷基化诱变：缺乏适应性反应的证据。
Curr Genet. 1986;10(9):647-55. doi: 10.1007/BF00410912.
2
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本文引用的文献

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Simultaneous induction of multiple mutations by N-methyl-N'-nitro-N-nitrosoguanidine in the yeast Saccharomyces cerevisiae.N-甲基-N'-硝基-N-亚硝基胍在酿酒酵母中同时诱导多个突变。
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Induction of S.cerevisiae MAG 3-methyladenine DNA glycosylase transcript levels in response to DNA damage.酿酒酵母MAG 3-甲基腺嘌呤DNA糖基化酶转录水平对DNA损伤的应答诱导。
Nucleic Acids Res. 1991 Dec 11;19(23):6427-32. doi: 10.1093/nar/19.23.6427.
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UV-induced mutability in repair-deficient rad6-1 strains of Saccharomyces cerevisiae is caused by a suppressor gene.酿酒酵母修复缺陷型rad6-1菌株中紫外线诱导的突变性是由一个抑制基因引起的。
Folia Microbiol (Praha). 1992;37(4):267-72. doi: 10.1007/BF02814561.
大鼠肝脏提取物对肝毒素的反应促进DNA中O6-甲基鸟嘌呤的甲基基团向蛋白质转移。
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Carcinogenesis. 1982;3(4):439-44. doi: 10.1093/carcin/3.4.439.
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Induction of a DNA glycosylase for N-methylated purines is part of the adaptive response to alkylating agents.诱导一种针对N-甲基化嘌呤的DNA糖基化酶是对烷化剂适应性反应的一部分。
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Role of the uvrE gene product and of inducible O6-methylguanine removal in the induction of mutations by N-methyl-N'-nitro-N-nitrosoguanidine in Escherichia coli.uvrE基因产物及诱导性O6-甲基鸟嘌呤去除在大肠杆菌中N-甲基-N'-硝基-N-亚硝基胍诱导突变中的作用。
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