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本文引用的文献

1
Diagnosing periprosthetic infection: false-positive intraoperative Gram stains.诊断假体周围感染：术中革兰氏染色假阳性。
Clin Orthop Relat Res. 2011 Apr;469(4):954-60. doi: 10.1007/s11999-010-1589-9.
2
Spondylodiscitis: update on diagnosis and management.脊柱 discs 炎：诊断和治疗的最新进展。
J Antimicrob Chemother. 2010 Nov;65 Suppl 3:iii11-24. doi: 10.1093/jac/dkq303.
3
Detection of methicillin-resistant Staphylococcus aureus (MRSA) in specimens from various body sites: performance characteristics of the BD GeneOhm MRSA assay, the Xpert MRSA assay, and broth-enriched culture in an area with a low prevalence of MRSA infections.检测来自不同身体部位标本中的耐甲氧西林金黄色葡萄球菌（MRSA）：BD GeneOhm MRSA 检测、Xpert MRSA 检测和肉汤增菌培养在 MRSA 感染低流行地区的性能特征。
J Clin Microbiol. 2010 Nov;48(11):3882-7. doi: 10.1128/JCM.00670-10. Epub 2010 Sep 22.
4
Recommendations for bone and joint prosthetic device infections in clinical practice (prosthesis, implants, osteosynthesis). Société de Pathologie Infectieuse de Langue Française.临床实践中骨与关节假体装置感染的建议（假体、植入物、骨接合术）。法国感染性病理学会。
Med Mal Infect. 2010 Apr;40(4):185-211. doi: 10.1016/j.medmal.2009.12.009. Epub 2010 Mar 19.
5
Detection of periprosthetic infections with use of ribosomal RNA-based polymerase chain reaction.基于核糖体 RNA 的聚合酶链反应检测假体周围感染。
J Bone Joint Surg Am. 2010 Mar;92(3):654-63. doi: 10.2106/JBJS.I.00400.
6
Improved diagnosis of periprosthetic joint infection by multiplex PCR of sonication fluid from removed implants.采用移除植入物的超声液的多重 PCR 提高假体周围关节感染的诊断。
J Clin Microbiol. 2010 Apr;48(4):1208-14. doi: 10.1128/JCM.00006-10. Epub 2010 Feb 17.
7
How common is MRSA in adult septic arthritis?耐甲氧西林金黄色葡萄球菌（MRSA）在成人脓毒性关节炎中有多常见？
Ann Emerg Med. 2009 Nov;54(5):695-700. doi: 10.1016/j.annemergmed.2009.06.511. Epub 2009 Aug 8.
8
Broad-range PCR in selected episodes of prosthetic joint infection.人工关节感染特定病例中的广谱聚合酶链反应
Infection. 2009 Jun;37(3):292-4. doi: 10.1007/s15010-008-8246-1. Epub 2009 Apr 23.
9
New real-time PCR-based method for Kingella kingae DNA detection: application to samples collected from 89 children with acute arthritis.基于新型实时聚合酶链反应的金氏金杆菌DNA检测方法：应用于89例急性关节炎患儿采集的样本
J Clin Microbiol. 2009 Jun;47(6):1837-41. doi: 10.1128/JCM.00144-09. Epub 2009 Apr 15.
10
Microbiologic diagnosis of prosthetic shoulder infection by use of implant sonication.通过植入物超声处理对人工肩关节感染进行微生物学诊断。
J Clin Microbiol. 2009 Jun;47(6):1878-84. doi: 10.1128/JCM.01686-08. Epub 2009 Mar 4.

利用 Xpert MRSA/SA SSTI 实时 PCR 直接检测骨关节炎感染金黄色葡萄球菌。

Direct detection of Staphylococcus osteoarticular infections by use of Xpert MRSA/SA SSTI real-time PCR.

机构信息

Laboratoire, Clinique de L'Union, Bd de Ratalens, 31240 St Jean, France.

出版信息

J Clin Microbiol. 2011 Dec;49(12):4225-30. doi: 10.1128/JCM.00334-11. Epub 2011 Oct 12.

DOI:10.1128/JCM.00334-11
PMID:21998420
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3232950/
Abstract

We evaluated the Xpert MRSA/SA SSTI real-time PCR assay (Cepheid, Sunnyvale, CA) directly on perioperative bone and joint samples. The sensitivity and specificity for detection of methicillin-susceptible Staphylococcus aureus (MSSA), methicillin-resistant Staphylococcus aureus (MRSA), and methicillin-resistant coagulase-negative Staphylococcus were, respectively, 100% and 98.3%, 100% and 100%, and 100% and 95.3%. The median total test turnaround time was 72 min for PCR versus 79 h for culture. Using these rapid results, appropriate antibiotic treatment could be rapidly initiated.

摘要

我们直接在围手术期骨和关节样本上评估了 Xpert MRSA/SA SSTI 实时 PCR 检测（Cepheid，加利福尼亚州森尼韦尔）。检测甲氧西林敏感金黄色葡萄球菌（MSSA）、耐甲氧西林金黄色葡萄球菌（MRSA）和耐甲氧西林凝固酶阴性葡萄球菌的灵敏度和特异性分别为 100%和 98.3%、100%和 100%以及 100%和 95.3%。与培养相比，PCR 的中位总检测周转时间为 72 分钟。利用这些快速结果，可以迅速开始进行适当的抗生素治疗。