利用 Xpert MRSA/SA SSTI 实时 PCR 直接检测骨关节炎感染金黄色葡萄球菌。
Direct detection of Staphylococcus osteoarticular infections by use of Xpert MRSA/SA SSTI real-time PCR.
机构信息
Laboratoire, Clinique de L'Union, Bd de Ratalens, 31240 St Jean, France.
出版信息
J Clin Microbiol. 2011 Dec;49(12):4225-30. doi: 10.1128/JCM.00334-11. Epub 2011 Oct 12.
Abstract
We evaluated the Xpert MRSA/SA SSTI real-time PCR assay (Cepheid, Sunnyvale, CA) directly on perioperative bone and joint samples. The sensitivity and specificity for detection of methicillin-susceptible Staphylococcus aureus (MSSA), methicillin-resistant Staphylococcus aureus (MRSA), and methicillin-resistant coagulase-negative Staphylococcus were, respectively, 100% and 98.3%, 100% and 100%, and 100% and 95.3%. The median total test turnaround time was 72 min for PCR versus 79 h for culture. Using these rapid results, appropriate antibiotic treatment could be rapidly initiated.
摘要
我们直接在围手术期骨和关节样本上评估了 Xpert MRSA/SA SSTI 实时 PCR 检测（Cepheid，加利福尼亚州森尼韦尔）。检测甲氧西林敏感金黄色葡萄球菌（MSSA）、耐甲氧西林金黄色葡萄球菌（MRSA）和耐甲氧西林凝固酶阴性葡萄球菌的灵敏度和特异性分别为 100%和 98.3%、100%和 100%以及 100%和 95.3%。与培养相比，PCR 的中位总检测周转时间为 72 分钟。利用这些快速结果，可以迅速开始进行适当的抗生素治疗。
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