Lee Nam-Jin, Lee Jung Won, Sung Jong Hwan, Lee Young-Jung, Kang Jong-Koo
College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National University, Cheongju, Korea.
Lab Anim Res. 2011 Sep;27(3):227-34. doi: 10.5625/lar.2011.27.3.227. Epub 2011 Sep 30.
IH-901 (20-O-β-D-glucopyranosyl-20(S)-protopanaxadiol or compound K) is a final intestinal bacterial metabolite of ginseng in humans. It has various pharmacologic effects such as antiaging, immunopotentiation, antistress, and antimetastatic activities. We analyzed the antioxidant activities of IH-901 using several assays including: total antioxidant activity, reductive potential, 1,1-diphenyl-2-picryl-hydrazyl, hydroxyl, superoxide and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging assays, a nitric oxide scavenging assay and a lipid peroxidation assay. At concentrations of 25 and 100 µg/mL, IH-901 inhibited lipid peroxidation of a linoleic acid emulsion with a potency comparable to ascorbic acid and butylated hydroxyanisole. The reductive potential of IH-901 increased in a concentration-dependent manner. IH-901 exhibited strong DPPH, hydroxyl, superoxide and ABTS radical scavenging activities. IH-901 was also an effective inhibitor of lipid peroxidation, although IH-901 had only a mild scavenging activity against nitric oxide. These results suggest that IH-901 may be a useful antioxidant agent against reactive oxygen species.
IH - 901（20 - O - β - D - 吡喃葡萄糖基 - 20(S) - 原人参二醇或化合物K）是人参在人体内的最终肠道细菌代谢产物。它具有多种药理作用，如抗衰老、增强免疫、抗应激和抗转移活性。我们使用多种检测方法分析了IH - 901的抗氧化活性，包括：总抗氧化活性、还原电位、1,1 - 二苯基 - 2 - 苦基肼、羟基、超氧阴离子和2,2'- 联氮 - 双 - (3 - 乙基苯并噻唑啉 - 6 - 磺酸)（ABTS）自由基清除检测、一氧化氮清除检测和脂质过氧化检测。在浓度为25和100μg/mL时，IH - 901抑制亚油酸乳液的脂质过氧化，其效力与抗坏血酸和丁基羟基茴香醚相当。IH - 901的还原电位呈浓度依赖性增加。IH - 901表现出较强的DPPH、羟基、超氧阴离子和ABTS自由基清除活性。尽管IH - 901对一氧化氮只有轻微的清除活性，但它也是脂质过氧化的有效抑制剂。这些结果表明，IH - 901可能是一种对抗活性氧的有用抗氧化剂。
