Wu Xiao-Fang, Ji Lei, Xu De-Shun, Han Jian-Kang, Shen Yue-Hua, Chen Li-Ping, Zha Yun-Feng, Tang Ren-Shu, Yao Wen-Ting
Huzhou Center for Disease Control and Prevention, Huzhou 313000, China.
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi. 2011 Dec;25(6):437-40.
To study the molecular characteristics of Noroviruses causing outbreaks of acute gastroenteritis in Huzhou.
From 2008 to 2010, total 119 fecal specimens collected from outbreaks of acute gastroenteritis were tested for Norovirus. Partial sequence of RNA dependent RNA polymerase (RdRp) of the positive samples were amplified by RT-PCR, then the PCR production were purified, sequenced and put into phylogenetic analysis.
50 of 119 specimens were positive for Norovirus by real-time RT-PCR. Out of those 50 Norovirus positive specimens, 9 were Norovirus Genogroup I (GI) positive, 35 were Norovirus Genogroup II (GII) positive, 6 was both Norovirus GI and GII positive. 12 PCR products for RdRp were selected for further studies on sequencing. Phylogenetic analysis revealed that the 5 GI norovirus isolates were belonged to genotype GI/2 and GI/3. Of the 7 GII norovirus isolates, 6 were belonged to genotype GII/4, 1 was belonged to genotype Glib.
Norovirus is a major cause of outbreaks of acute gastroenteritis in Huzhou and the epidemic strains of norovirus isolated from Huzhou had a high degree of genetic diversity.
研究引起湖州市急性胃肠炎暴发的诺如病毒的分子特征。
2008年至2010年，对从急性胃肠炎暴发疫情中收集的119份粪便标本进行诺如病毒检测。对阳性样本的RNA依赖RNA聚合酶（RdRp）部分序列进行RT-PCR扩增，然后对PCR产物进行纯化、测序并进行系统发育分析。
119份标本中50份经实时RT-PCR检测诺如病毒呈阳性。在这50份诺如病毒阳性标本中，9份诺如病毒基因组I（GI）阳性，35份诺如病毒基因组II（GII）阳性，6份诺如病毒GI和GII均阳性。选择12份RdRp的PCR产物进行进一步测序研究。系统发育分析显示，5株GI诺如病毒分离株属于GI/2和GI/3基因型。7株GII诺如病毒分离株中，6株属于GII/4基因型，1株属于Glib基因型。
诺如病毒是湖州市急性胃肠炎暴发的主要原因，从湖州市分离出的诺如病毒流行株具有高度的遗传多样性。
