Department of Agricultural Biotechnology, University of Padova, Agripolis, Viale dell'Università 16, 35020 Legnaro, Italy.
J Agric Food Chem. 2011 Nov 23;59(22):11940-8. doi: 10.1021/jf202473e. Epub 2011 Nov 2.
Lignosulfonate-humate a and lignosulfonate-humate b, derived by an industrial process from lignin, were studied chemically and biologically, and their effects on maize metabolism compared with the responses induced by humic substances obtained from leonardite. Lignosulfonate-humate a and lignosulfonate-humate b elicited hormonelike activity and leonardite displayed giberellin properties. To improve our understanding of their biological action, lignosulfonate-humate a, lignosulfonate-humate b and leonardite were supplied to maize plants and their effect was studied on growth, nitrogen metabolism and photosynthesis. All products increased root and leaf growth. Glutamine-synthetase, glutamate-synthase enzyme activities and protein content were all increased. The treatments also increased chlorophyll content, glucose, fructose and rubisco enzyme activity, suggesting a positive role of lignosulfonate-humate a, lignosulfonate-humate b and leonardite in the photosynthetic process. In addition, an increase in phenol content was observed. In light of these results, being environmentally friendly products, lignosulfonate-humate a and lignosulfonate-humate b could be used to increase crop yield.
木质素磺酸盐-腐殖酸 a 和木质素磺酸盐-腐殖酸 b，通过工业过程从木质素中提取，进行了化学和生物学研究，并与从风化煤中获得的腐殖物质引起的反应进行了比较，研究了它们对玉米代谢的影响。木质素磺酸盐-腐殖酸 a 和木质素磺酸盐-腐殖酸 b 表现出激素样活性，风化煤显示出赤霉素特性。为了提高我们对其生物作用的理解，向玉米植株提供木质素磺酸盐-腐殖酸 a、木质素磺酸盐-腐殖酸 b 和风化煤，并研究它们对生长、氮代谢和光合作用的影响。所有产品都增加了根和叶的生长。谷氨酰胺合成酶、谷氨酸合酶酶活性和蛋白质含量均增加。处理还增加了叶绿素含量、葡萄糖、果糖和 Rubisco 酶活性，表明木质素磺酸盐-腐殖酸 a、木质素磺酸盐-腐殖酸 b 和风化煤在光合作用过程中具有积极作用。此外，还观察到酚类物质含量增加。鉴于这些结果，作为环保产品，木质素磺酸盐-腐殖酸 a 和木质素磺酸盐-腐殖酸 b 可用于提高作物产量。
