Urology Center, Southwest Hospital, Third Military Medical University, Chongqing, China.
Urology. 2011 Dec;78(6):1443.e1-6. doi: 10.1016/j.urology.2011.08.019. Epub 2011 Oct 13.
To explore the roles of stem cell factor (SCF) on the loss of interstitial cells (ICCs) in the bladder of diabetic rats, which have not been investigated.
The rats were assigned to 3 groups: normal control rats, diabetic rats, and SCF-treated diabetic rats. The diabetic rat model was created using a streptozotocin (60 mg/kg) intraperitoneal injection. The SCF and c-kit levels in bladder tissue were determined using reverse transcriptase-polymerase chain reaction and Western blot analysis. The quantity of ICCs as represented by c-kit-positive cells was examined by image analysis of immunofluorescence staining.
Compared with the control rats, the diabetic rats exhibited a significant decrease in the SCF levels and c-kit expression and number of ICCs in the bladder tissues. All these impaired parameters were effectively restored to the control level after exogenous SCF treatment.
These findings suggest that the loss of ICCs in the bladder tissue of diabetic rats can be attributed to a deficiency in endogenous SCF. The beneficial effect of exogenous SCF on diabetic depletion of ICCs could provide a theoretical rationale for the use of SCF as a potential therapeutic drug in treating patients with diabetes-related voiding dysfunction.
探讨干细胞因子（SCF）在糖尿病大鼠膀胱间质细胞（ICCs）丢失中的作用，目前尚未对此进行研究。
将大鼠分为 3 组：正常对照组大鼠、糖尿病组大鼠和 SCF 治疗的糖尿病组大鼠。采用链脲佐菌素（60mg/kg）腹腔注射建立糖尿病大鼠模型。采用逆转录-聚合酶链反应和 Western blot 分析检测膀胱组织中 SCF 和 c-kit 水平。通过免疫荧光染色的图像分析检测 c-kit 阳性细胞代表的 ICC 数量。
与对照组大鼠相比，糖尿病大鼠的膀胱组织中 SCF 水平和 c-kit 表达以及 ICC 数量显著降低。外源性 SCF 治疗后，所有这些受损参数均有效恢复至对照水平。
这些发现表明，糖尿病大鼠膀胱组织中 ICC 的丢失可能归因于内源性 SCF 的缺乏。外源性 SCF 对糖尿病大鼠 ICC 耗竭的有益作用为将 SCF 用作治疗糖尿病相关排尿功能障碍患者的潜在治疗药物提供了理论依据。
