Jiang Nan, Yu Yue, Chen Feng-Qin, Yuan Li-Li, Wang Qiao-Min
Department of Gastroenterology, Affiliated Provincial Hospital, Anhui Medical University, Hefei 230001, China.
Zhongguo Zhong Xi Yi Jie He Za Zhi. 2013 Dec;33(12):1672-6.
To observe the effect of Banxia Xiexin Decoction (BXD) on expression of the interstitial cells of Cajal (ICCs) and stem cell factor (SCF) in the antrum of rats with diabetes mellitus (DM).
Totally sixty healthy male SD rats were randomly divided into the control group, the model group, the BXD group, and the Western medicine group (WM, treated by domperidone), 15 in each group. Diabetic rat models were established by a single intraperitoneal injection of streptozotocin (STZ, 55 mg/kg). Those in the BXD group were perfused with BXD at the daily dose of 5.4 g/kg. An equal volume of distilled water was given by gastrogavage to those in the WM group and the control group for 8 successive weeks. The body weight and blood glucose of all rats were detected, and the gastric residual rates were detected with semisolid nutrient paste by gastrogavage. The expression of positive ICCs and SCF were observed by immunohistochemical method and quantified image analyzer.
Compared with the control group,the body weight reduced, blood glucose and gastric residual rates increased, and the mean optical density of positive ICCs and SCF significantly decreased in the model group (P < 0.05). Compared with the model group,symptoms such as polydipsia, polyphagia, polyuria were relieved, spirits improved, the body weight and mean optical densities of positive ICCs and SCF significantly increased (P < 0.05), and gastric residual rates significantly decreased in the BXD group and the WM group (P < 0.05). The blood glucose significantly decreased (P < 0.05) in the BXD group. The mean optical density of positive ICCs was higher in the BXD group than in the WM group (P < 0.05).
BXD could promote the expression of positive ICCs and SCF. It could improve the gastric motility in DM rats by partially inverting abnormal changes of gastric antral ICCs and SCF.
观察半夏泻心汤对糖尿病大鼠胃窦Cajal间质细胞（ICCs）及干细胞因子（SCF）表达的影响。
将60只健康雄性SD大鼠随机分为对照组、模型组、半夏泻心汤组和西药组（多潘立酮治疗），每组15只。采用腹腔注射链脲佐菌素（STZ，55mg/kg）建立糖尿病大鼠模型。半夏泻心汤组按每日5.4g/kg的剂量灌胃给予半夏泻心汤。西药组和对照组给予等体积蒸馏水连续灌胃8周。检测所有大鼠的体重和血糖，采用半固体营养糊灌胃检测胃残留率。采用免疫组化法和图像定量分析仪观察ICCs和SCF阳性表达情况。
与对照组比较，模型组大鼠体重降低，血糖和胃残留率升高，ICCs和SCF阳性平均光密度显著降低（P<0.05）。与模型组比较，半夏泻心汤组和西药组大鼠多饮、多食、多尿等症状缓解，精神状态改善，体重及ICCs和SCF阳性平均光密度显著升高（P<0.05），胃残留率显著降低（P<0.05）。半夏泻心汤组血糖显著降低（P<0.05）。半夏泻心汤组ICCs阳性平均光密度高于西药组（P<0.05）。
半夏泻心汤可促进ICCs和SCF阳性表达，通过部分逆转糖尿病大鼠胃窦ICCs和SCF的异常改变改善胃动力。
