仅涉及肉汤培养和血清学反应的干制食品和饲料中沙门氏菌检测的加速程序。
Accelerated procedure for Salmonella detection in dried foods and feeds involving only borth cultures and serological reactions.
作者信息
Sperber W H, Deibel R H
出版信息
Appl Microbiol. 1969 Apr;17(4):533-9. doi: 10.1128/am.17.4.533-539.1969.
Abstract
A procedure has been developed in which Salmonella can be detected in dried foods and feeds within 50 hr. This includes preenrichment (18 hr), selective enrichment (24 hr), elective enrichment (6 to 8 hr), and serological testing (2 hr). The procedure is as sensitive as the more time-consuming, traditional (plating, biochemical, and serological) procedure which may require at least 4 to 5 days. The new procedure is rapid and accurate in comparison to traditional procedures. Moreover, it is simple and inexpensive to perform. As described, the procedure does not necessitate the isolation of pure cultures, but this step is performed easily if desired.
摘要
已开发出一种方法，可在50小时内检测出干燥食品和饲料中的沙门氏菌。这包括预富集（18小时）、选择性富集（24小时）、选择性增菌（6至8小时）和血清学检测（2小时）。该方法与可能需要至少4至5天的耗时更长的传统（平板培养、生化和血清学）方法一样灵敏。与传统方法相比，新方法快速且准确。此外，该方法操作简单且成本低廉。如所述，该方法无需分离纯培养物，但如果需要，这一步骤也很容易完成。
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