Bosslet K, Keweloh H C, Hermentin P, Muhrer K H, Sedlacek H H, Schulz G
Research Laboratories of Behringwerke AG, Marburg, FRG.
Br J Cancer Suppl. 1990 Jul;10:37-9.
The distribution of the monoclonal antibody (MAb) BW494 in human pancreatic carcinoma biopsies during high dose intravenous immunotherapy was investigated. Using immunohistochemical techniques combined with anti-idiotypic, endothelial cell-specific and bispecific MAbs, it was shown that 3 days after onset of immunotherapy, MAb BW494 was bivalently bound to tumour cells in some highly vascularised areas near capillaries. No binding was observed in other highly vascularised tumour cell areas although the epitope detected by MAb BW494 was present. In contrast to our expectation the majority of the tumour cells were not yet saturated by the antibody, probably due to diffusion barriers in the solid tumour tissue.
研究了高剂量静脉免疫治疗期间单克隆抗体（MAb）BW494在人胰腺癌活检组织中的分布。采用免疫组化技术结合抗独特型、内皮细胞特异性和双特异性单克隆抗体，结果显示免疫治疗开始3天后，MAb BW494在毛细血管附近一些高度血管化区域与肿瘤细胞发生双价结合。尽管存在MAb BW494检测到的表位，但在其他高度血管化的肿瘤细胞区域未观察到结合。与我们的预期相反，大多数肿瘤细胞尚未被抗体饱和，这可能是由于实体瘤组织中的扩散屏障所致。
