一种对人胰腺癌细胞具有结合和抑制活性的单克隆抗体。I. 一种针对胰腺高分化腺癌筛选出的鼠单克隆抗体的免疫组织化学和免疫化学特征。
A monoclonal antibody with binding and inhibiting activity towards human pancreatic carcinoma cells. I. Immunohistological and immunochemical characterization of a murine monoclonal antibody selecting for well differentiated adenocarcinomas of the pancreas.
作者信息
Bosslet K, Kern H F, Kanzy E J, Steinstraesser A, Schwarz A, Lüben G, Schorlemmer H U, Sedlacek H H
出版信息
Cancer Immunol Immunother. 1986;23(3):185-91. doi: 10.1007/BF00205648.
Abstract
The murine monoclonal antibody (MAb) BW 494 was characterized in relation to its tissue specificity, the epitope recognized, in vitro and in vivo radiolocalization and its potential to mediate antibody dependent cellular cytotoxicity (ADCC) and complement mediated cytolysis (CMC). The MAb defined carbohydrate epitope located on a greater than 200 k daltons glycoprotein was mainly expressed on the majority of well differentiated adenocarcinomas of the pancreas. Furthermore, the epitope is accessible to MAb BW 494 in vivo, allowing an enrichment of radioactive antibody at the tumor site in nude mice. Additionally, MAb BW 494 is able to use human peripheral blood lymphocytes as effector cells for ADCC reactions against appropriate tumor target cells in vitro. In contrast, the antibody does not mediate human or rabbit CMC.
摘要
对鼠单克隆抗体（MAb）BW 494进行了特性研究，涉及组织特异性、所识别的表位、体内外放射性定位以及介导抗体依赖性细胞毒性（ADCC）和补体介导的细胞溶解（CMC）的潜力。该单克隆抗体所定义的位于大于200千道尔顿糖蛋白上的碳水化合物表位主要表达于大多数高分化胰腺腺癌。此外，该表位在体内可被单克隆抗体BW 494识别，使得放射性抗体在裸鼠肿瘤部位富集。另外，单克隆抗体BW 494能够利用人外周血淋巴细胞作为效应细胞，在体外针对合适的肿瘤靶细胞进行ADCC反应。相比之下，该抗体不介导人或兔的CMC。
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