Department of Physiology, University of Saskatchewan, Saskatoon, SK, Canada.
Learn Mem. 2011 Oct 17;18(11):688-92. doi: 10.1101/lm.2312711. Print 2011 Nov.
Mechanisms consistent with long-term depression in the perirhinal cortex (PRh) play a fundamental role in object recognition memory; however, whether AMPA receptor endocytosis is involved in distinct phases of recognition memory is not known. To address this question, we used local PRh infusions of the cell membrane-permeable Tat-GluA2(3Y) interference peptide or a scrambled control to block the endocytosis of AMPA receptors during the encoding, consolidation, or retrieval phase of object recognition memory. Tat-GluA2(3Y) infusion before the encoding and consolidation phases did not alter recognition memory. In contrast, Tat-GluA2(3Y) infusion prior to the retrieval phase disrupted object recognition memory. The present results indicate a distinct role for AMPA receptor endocytosis in the retrieval of visual recognition memory.
在边缘区（PRh）中与长期抑郁相一致的机制在物体识别记忆中起着至关重要的作用；然而，AMPA 受体内吞作用是否参与识别记忆的不同阶段尚不清楚。为了解决这个问题，我们使用局部 PRh 输注可穿透细胞膜的 Tat-GluA2(3Y)干扰肽或随机对照肽来阻断 AMPA 受体在物体识别记忆的编码、巩固或检索阶段的内吞作用。编码和巩固阶段之前的 Tat-GluA2(3Y)输注不会改变识别记忆。相比之下，在检索阶段之前的 Tat-GluA2(3Y)输注会破坏物体识别记忆。本研究结果表明，AMPA 受体内吞作用在视觉识别记忆的检索中具有独特的作用。
