Latorre Rafael, Hernández Wendy, Sun Fei, Sánchez-Margallo Francisco, Gil Francisco, López-Albors Octavio, Vázquez Jose M, Usón Jesús
Anatomy and Embryology, Veterinary Faculty, University of Murcia, Campus de Espinardo, 30100 Murcia, Spain.
Exp Diabetes Res. 2011;2011:976910. doi: 10.1155/2011/976910. Epub 2011 Oct 4.
To prospectively evaluate the technical feasibility and efficacy of wedged arterial injection (WAI) as a potential route for experimental selective therapy to the pancreas of healthy pigs.
Selective angiographies were completed in ten pigs under general anaesthesia. By superselective angiography, the catheter was inserted and wedged into the major pancreatic artery, blocking the blood flow. In order to evaluate the efficacy of the WAI method, a DNA-specific fluorescent dye (Hoechst 33258) was used.
Histological study revealed a uniform distribution of the fluorescent dye within the nuclei of the endocrine and exocrine pancreatic cells. Pancreatic and liver enzymes as well as histopathology of the pancreas were normal.
WAI is a highly effective minimally invasive methodology to target the porcine pancreas. The findings suggest that WAI may contribute to developing preclinical assays of pancreas gene or cell-transfer therapies in swine model.
前瞻性评估楔形动脉注射（WAI）作为健康猪胰腺实验性选择性治疗潜在途径的技术可行性和疗效。
对10头猪进行全身麻醉下的选择性血管造影。通过超选择性血管造影，将导管插入并楔入胰腺主要动脉，阻断血流。为评估WAI方法的疗效，使用了一种DNA特异性荧光染料（Hoechst 33258）。
组织学研究显示荧光染料在内分泌和外分泌胰腺细胞核内均匀分布。胰腺和肝脏酶以及胰腺组织病理学均正常。
WAI是一种针对猪胰腺的高效微创方法。研究结果表明，WAI可能有助于在猪模型中开展胰腺基因或细胞移植治疗的临床前试验。
